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促肝细胞生长素对大鼠肝缺血再灌注损伤的保护作用及其机制探讨
Protective effect of hepatocyte growth-promoting factor against liver ischemia-reperfusion injury in rats and its mechanism of action
文章发布日期:2019年01月07日  来源:  作者:罗燕青,冯春林,梅永,等  点击次数:149次  下载次数:15次

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【摘要】:目的 探讨促肝细胞生长素(PHGF)对大鼠肝缺血再灌注损伤的保护作用及机制。方法 将80只健康雄性SD大鼠随机分为实验组(PHGF组)和对照组(NS组),每组各40只。通过阻断肝中叶及肝左叶血流造成70%肝脏缺血建立大鼠肝缺血再灌注损伤模型,缺血时间为21 min。PHGF组术前予PHGF腹腔注射干预,NS组术前给予等量生理盐水。术前及术后1、3、5、7 d取大鼠血清及肝组织,检测ALT、AST、TBil水平;HE染色观察病理改变;real-time PCR检测肝组织线粒体转录因子A(TFAM)mRNA水平。计量资料两组间比较采用独立样本t检验。结果 HE染色结果显示,光镜下PHGF组肝细胞水肿、炎细胞浸润程度及肝细胞坏死程度较NS组明显减轻。肝功能生化检测结果显示,PHGF组大鼠术后1、3、5、7 d血清ALT、AST、TBil水平低于同时相NS组,差异均有统计学意义(t值分别为11.879、16.019、22.168、10.235、9.041、12.936、18.759、8.142、10.108、11.014、13.245、9.968,P值均<0.001)。real-time PCR结果显示,PHGF组大鼠术后1、3、5 d肝组织TFAM mRNA水平较NS组明显升高(t值分别为7.998、14.764、13.861,P值均<0.001)。结论 PHGF预处理对大鼠肝缺血再灌注损伤具有保护作用,其作用机制可能是通过上调TFAM表达减轻肝缺血再灌注损伤。
【Abstract】:Objective To investigate the protective effect of hepatocyte growth-promoting factor (PHGF) against liver ischemia-reperfusion injury in rats and its mechanism of its action. Methods A total of 80 healthy male Sprague-Dawley rats were randomly divided into experimental group (PHGF group) and control group (NS group), with 40 rats in each group. A rat model of liver ischemia-reperfusion injury was established by 70% liver ischemia caused by the occlusion of blood flow in the middle and left lobes of the liver, with an ischemia time of 21 minutes. The rats in the PHGF group were given intraperitoneal injection of PHGF for intervention before surgery, and those in the NS group were given an equal volume of normal saline. Serum and liver tissue samples were collected before surgery and on days 1, 3, 5, and 7 after surgery, and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and total bilirubin (TBil) were measured; HE staining was used to observe pathological changes; real-time PCR was used to measure the mRNA expression of mitochondrial transcription factor A (TFAM) in the liver. The independent samples t-test was used for comparison of continuous data between two groups. Results HE staining showed that compared with the NS group, the PHGF group had significantly lower degrees of hepatocyte swelling, inflammatory cell infiltration, and hepatocyte necrosis under a light microscope. Liver biochemistry showed that on days 1, 3, 5, and 7 after surgery, the PHGF group had significantly lower serum levels of ALT, AST, and TBil than the NS group (t=11.879, 16.019, 22168, 10.235, 9.041, 12.936, 18.759, 8.142, 10.108, 11.014, 13.245, and 9.968, all P<0.001). Real-time PCR showed that on days 1, 3, and 5 after surgery, the PHGF group had a significantly higher mRNA level of TFAM in the liver than the NS group (t=7.998, 14.764, and 13.861, all P<0.001). Conclusion PHGF preconditioning exerts a protective effect against liver ischemia-reperfusion injury in rats, possibly by upregulating the expression of TFAM to alleviate liver ischemia-reperfusion injury.
【关键字】:再灌注损伤; 促肝细胞生长素; 线粒体转录因子A; 大鼠, Sprague-Dawley
【Key words】:reperfusion injury; hepatocyte growth-promoting factor; mitochondrial transcription factor A; rats, Sprague-Dawley
【引证本文】:LUO YQ, FENG CL, MEI Y, et al. Protective effect of hepatocyte growth-promoting factor against liver ischemia-reperfusion injury in rats and its mechanism of action[J]. J Clin Hepatol, 2019, 35(2): 364-367. (in Chinese)
罗燕青,冯春林,梅永,等. 促肝细胞生长素对大鼠肝缺血再灌注损伤的保护作用及其机制探讨[J]. 临床肝胆病杂志, 2019, 35(2): 364-367.

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