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不同途径移植骨髓干细胞治疗急性肝损伤小鼠模型的效果比较
Clinical effects of bone marrow stem cell transplantation through different approaches in mice with acute liver injury
文章发布日期:2018年12月17日  来源:  作者:穆丽雅,唐秀芬,李淑芹,等  点击次数:246次  下载次数:46次

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【摘要】:目的探讨4种途径移植骨髓干细胞治疗的急性肝损伤小鼠的肝脏迁移情况及肝损伤的修复情况。方法雄性BALB/c小鼠分为A、B、C、D、E、F 6组,每组10只,A、B、C、D为移植组,E组为骨髓干细胞供体组,F组为急性肝损伤模型组。用CCL4 /2-乙酰氨基芴制备小鼠急性肝损伤模型,分离小鼠骨髓干细胞,用红色荧光染料PKH26标记后经门静脉(A组,n=10)、尾静脉(B组,n=10)、腹腔(C组,n=10)及脾内(D组,n=10)输入到急性肝损伤小鼠体内,2周后处死小鼠,血清检测肝功能(ALT、AST、Alb),肝组织病理观察骨髓干细胞向肝脏迁移的情况及肝损伤小鼠的肝脏修复情况。F组小鼠于第8天处死检测ALT、AST及Alb值。计量资料2组间比较采用t检验,多组间比较采用单因素方差分析。结果显微镜下4个移植组移植的细胞均迁移到肝脏且通过病理图片均可见新生的肝细胞;ALT、AST、Alb值A、B、C、D 4组分别与F组比较差异均有统计学意义(ALT:t值分别为2.372、2.473、2.354、2.383,P值均<0.05;AST:t值分别为2.534、2.423、2.437、2.643,P值均<0.05;Alb:t值分别为2.336、2.243、2.373、2.352,P值均<0.05)。结论骨髓干细胞促进急性肝损伤小鼠肝脏的修复,其修复程度与移植途径无关。
【Abstract】:ObjectiveTo investigate the migration of bone marrow stem cells (BMSCs) to the liver and liver repair in mice with acute liver injury treated with BMSC transplantation through four approaches. MethodsMale BALB/c mice were divided into groups A, B, C, D, E, and F, with 10 mice in each group. Groups A, B, C, and D were treated by transplantation, group E was used as the donor of BMSCs, and group F was used as the model of acute liver injury. CCL4/2-AFF was used to establish the model of acute liver injury. Mouse BMSCs were isolated, labeled with the red fluorescent dye PKH26, and then transplanted into the mice with acute liver injury through the portal vein (group A), the tail vein (group B), the abdominal cavity (group C), and the spleen (group D). The mice were sacrificed 2 weeks later. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and albumin (Alb) were measured. The pathology of liver tissue was observed to evaluate the migration of BMSCs to the liver and the degree of liver repair. The mice in group F were sacrificed on day 8 to measure the levels of ALT, AST, and Alb. The t test was used for comparison of continuous data between two groups, and one-way analysis of variance was used for comparison of continuous data between multiple groups. ResultsIn groups A, B, C, and D, transplanted BMSCs migrated to the liver under a microscope, and newly formed hepatocytes were observed on pathological images. There were significant differences in the levels of ALT, AST, and Alb between groups A, B, C, and D and group F (ALT: t=2.372, 2.473, 2.354, and 2.383, all P<0.05; AST: t=2.534, 2.423, 2.437, and 2.643, all P<0.05; Alb: t=2.336, 2.243, 2.373, and 2.352, all P<0.05). ConclusionBMSCs can promote repair of the liver in mice with acute liver injury, and the degree of liver repair is not related to the transplantation approach.
【关键字】:肝疾病; 骨髓移植; 动物实验; 治疗结果; 小鼠, 近交BALB C
【Key words】:liver diseases; bone marrow transplantation; animal experimentation;treatment outcome; mice, inbred BALB C
【引证本文】:MU LY, TANG XF, LI SQ, et al. Clinical effects of bone marrow stem cell transplantation through different approaches in mice with acute liver injury[J]. J Clin Hepatol, 2019, 35(1): 153-156. (in Chinese)
穆丽雅, 唐秀芬, 李淑芹, 等. 不同途径移植骨髓干细胞治疗急性肝损伤小鼠模型的效果比较[J]. 临床肝胆病杂志, 2019, 35(1): 153-156.

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