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肝细胞癌中microRNA-888基因家族的表达特征及临床意义
Expression features and clinical significance of the microRNA-888 gene family in hepatocellular carcinoma
文章发布日期:2018年12月17日  来源:  作者:黄湘俊,张文兴  点击次数:259次  下载次数:50次

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【摘要】:目的初步探讨microRNA-888(miRNA-888)基因家族成员在肝细胞癌中的表达特征及其意义。方法选取湖南中医药大学第一附属医院在2012年1月-2017年12月病理确诊为肝细胞癌的患者72例,另收集72例健康自愿者的肝组织作为正常对照组。运用实时定量PCR和原位杂交分析肝癌组织中miRNA-888家族中miRNA-888、miRNA-891a、miRNA-891b、miRNA-892a和miRNA-892b的表达情况,初步探讨miRNA-888、miRNA-891b和miRNA-892a的表达与肝癌临床病理特征的关系。计量资料2组间比较采用t检验; 计数资料组间比较采用χ 2检验。结果miRNA-888、miRNA-891b、miRNA-892a在肝癌组织中的表达明显高于正常肝组织( 2.53±0.75 vs 0.46±0.08, t=14.02,P<0.001; 2.26±0.38 vs 1.19±0.21, t=7.75, P<0.001; 5.44±1.01 vs 1.06±0.30, t=35.27, P<0.001);原位杂交分析进一步验证了miRNA-888、miRNA-891b和 miRNA-892a 3种miRNAs主要表达定位于肝癌组织中的细胞核,与正常肝组织表达水平比较差异均有统计学意义 (3.91±0.92 vs 1.21±0.42, t=2265, P<0.001; 2.92±0.76 vs 0.83±0.21, t=22.92, P<0.001; 3.81±0.99 vs 1.30±0.32, t=20.47,P<0.001)。miRNA-888和miRNA-891b高表达在组织学分级和临床分期的比较中差异有统计学意义(χ2值分别为6.25、4.44、4.76、6.05,P值均<0.05),而miRNA-892a高表达在Ⅲ、Ⅳ期肿瘤中表达更明显(χ2=8.50,P<0.001)。结论miRNA-888、miRNA-891b及miRNA-892a在肝癌组织中表达明显增加;miRNA-888,miRNA-891b和miRNA-892a表达升高与肝癌恶性程度更高密切相关。
【Abstract】:ObjectiveTo investigate the expression features and clinical significance of the microRNA-888 (miRNA-888) gene family members in hepatocellular carcinoma (HCC). MethodsA total of 72 patients with pathologically confirmed HCC who were treated in The First Affiliated Hospital of Hunan University of Chinese Medicine from January 2012 to December 2017 were enrolled, and 72 liver tissue samples collected from healthy volunteers were enrolled as normal control group. Quantitative real-time PCR and in situ hybridization were used to measure the expression of miRNA-888 family members (miRNA-888, miRNA-891a, miRNA-891b, miRNA-892a, and miRNA-892b) in HCC tissue, and the association of the expression of miRNA-888, miRNA-891b, and miRNA-892a with the clinicopathological features of HCC was analyzed. The t-test was used for comparison of continuous data between two groups, and the chi-square test was used for comparison of categorical data between two groups. ResultsHCC tissue had significantly higher expression of miRNA-888, miRNA-891b, and miRNA-892a than normal liver tissue (miRNA-888: 2.53±0.75 vs 0.46±0.08, t=14.02, P<0.001; miRNA-891b: 2.26±0.38 vs 1.19±0.21, t=7.75, P<0.001; miRNA-892a: 5.44±1.01 vs 1.06±0.30, t=35.27, P<0.001). According to the results of in situ hybridization, the miRNAs of miRNA-888, miRNA-891b, and miRNA-892a were mainly located in the cell nucleus of HCC tissue, and there were significant differences in the expression of these miRNAs between HCC tissue and normal tissue (miRNA-888: 3.91±0.92 vs 1.21±0.42, t=22.65, P<0.001; miRNA-891b: 2.92±0.76 vs 0.83±0.21, t=22.92, P<0.001; miRNA-892a: 3.81±0.99 vs 1.30±0.32, t=20.47, P<0001). There were significant differences in the expression of miRNA-888 and miRNA-891b between the patients with different histological grades and clinical stages (χ2=6.25, 4.44, 4.76, and 6.05, all P<0.05), and there was a significantly higher expression of miRNA-892a in stage III/IV tumors (χ2=8.50, P<0.001). ConclusionThere are significant increases in the expression of miRNA-888, miRNA-891b, and miRNA-892a in HCC tissue, and such increases are closely associated with the malignancy of HCC.
【关键字】:癌, 肝细胞; 微RNAs; 基因表达
【Key words】:carcinoma, hepatocellular; micrornas; gene expression
【引证本文】:HUANG XJ, ZHANG WX. Expression features and clinical significance of the microRNA-888 gene family in hepatocellular carcinoma[J]. J Clin Hepatol, 2019, 35(1): 119-122. (in Chinese)
黄湘俊, 张文兴. 肝细胞癌中microRNA-888基因家族的表达特征及临床意义[J]. 临床肝胆病杂志,2019,35(1):119-122.

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