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MBOAT7 rs641738单核苷酸多态性与非酒精性脂肪性肝病的相关性
Association between MBOAT7 rs641738 polymorphism and nonalcoholic fatty liver disease
文章发布日期:2018年09月10日  来源:  作者:廖宋龄,刘守胜,王聪,等  点击次数:310次  下载次数:20次

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【摘要】:目的探讨我国青岛地区汉族人群中MBOAT7 rs641738位点多态性与非酒精性脂肪性肝病(NAFLD)发生的相关性,为NAFLD及其并发症的防治提供理论依据。方法纳入2017年4月-12月青岛市市立医院收入院的NAFLD患者113例,健康对照者74例。收集受试者的血液标本,进行DNA的提取,应用PCR及飞行时间质谱方法(MALDI-TOF MS)检测MBOAT7基因rs641738位点的基因型。应用χ2检验分析基因型的分布是否符合 Hardy-Weinberg平衡法则,以确定检验样本是否具有群体代表性。同时收集受试者的临床资料及实验室肝功能等生化检查结果。2组间性别、基因型及等位基因频率比较应用χ2检验进行分析。符合正态分布的计量资料2组间比较采用t检验,不符合正态分布的2组间比较采用Wilcoxon秩和检验。基因多态性与 NAFLD 发生的相对风险度以比值比(OR)及其95%可信区间(95%CI)表示,应用非条件logistic回归模型计算OR 及95%CI。结果 MBOAT7 rs641738位点CC、CT、TT基因型分布在NAFLD组与对照组间无明显差异(χ2=0.157,P=0.692),C、T等位基因的分布频率在2组之间亦无明显统计学差异(χ2=0.365,P=0.546)。应用非条件logistic回归模型分析结果示MBOAT7 rs641738位点CC+CT携带者与CC携带者相比,发病风险未增加(OR=0.923,95%CI:0.685~1.245,P=0.692);调整性别、年龄、BMI后,差异仍无统计学意义(校正OR=0.893,95%CI:0652~1.223,P=0.481)。在NAFLD组中,CT+TT基因型携带者TC、LDL水平明显低于CC基因型携带者(t值分别为-2.348、-2.113,P值分别为0.021、0.037),其余指标差异均无统计学意义(P值均>0.05)。结论MBOAT7 rs641738位点多态性在我国北方青岛地区汉族人群中与NAFLD的发病无明显相关性。
【Abstract】:ObjectiveTo investigate the association between MOBAT7 rs641738 polymorphism and the development of NAFLD in the Chinese Han population in Qingdao, China, and to provide a theoretical basis for the prevention and treatment of NAFLD and its complication. MethodsA total of 113 NAFLD patients who were treated in Qingdao Municipal Hospital and 74 healthy controls were enrolled in this study. Blood samples were collected, DNA was extracted, and PCR and time-of-flight mass spectrometry were used to determine the genotype of MBOAT7 rs641738. The chi-square test was used to determine whether the distribution of genotypes met the Hardy-Weinberg equilibrium, in order to assess the group representativeness of samples. Clinical data and biochemical results including liver function were collected. The chi-square test was used for comparison of sex and genotype and allele frequencies between the two groups. The independent samples t-test and Wilcoxon rank sum test were used for comparison of continuous data between the two groups. The relative risk of NAFLD associated with gene polymorphisms was expressed as odds ratio (OR) and 95% confidence interval (CI), and the non-conditional logistic regression model was used to calculate OR and 95%CI. ResultsThere was no significant difference in the distribution of CC, CT, and TT genotypes of MBOAT7 rs641738 between the NAFLD group and the control group (χ2=0.157, P=0.692), and there was also no significant difference in the distribution of C and T alleles between the two groups (χ2=0.365, P=0.546). The non-conditional logistic regression analysis showed that the carriers with CC+CT genotypes of MBOAT7 rs641738 had a similar risk of NAFLD as those with CC genotype (OR=0.923, 95%CI: 0.685-1.245, P=0.692); there was also no significant difference after adjustment for sex, age, and body mass index (corrected OR=0.893, 95%CI: 0.652-1.223, P=0.481). In the NAFLD group, the carriers with CC+CT genotypes had significantly lower levels of total cholesterol and low-density lipoprotein than those with CC genotype (t=-2.348 and -2.113, P=0.021 and 0.037), and there were no significant differences in the other indices (all P>0.05). ConclusionThere is no significant association between MBOAT7 rs641738 polymorphism and the risk of NAFLD in the Chinese Han population in Qingdao.
【关键字】:非酒精性脂肪性肝病; 多态性, 单核苷酸; MBOAT7
【Key words】:non-alcoholic fatty liver disease; polymorphism, single nucleotide; MBOAT7
【引证本文】:LIAO SL, LIU SS, WANG C, et al. Association between MBOAT7 rs641738 polymorphism and nonalcoholic fatty liver disease[J]. J Clin Hepatol, 2018, 34(10): 2169-2174. (in Chinese)
廖宋龄, 刘守胜, 王聪, 等. MBOAT7 rs641738单核苷酸多态性与非酒精性脂肪性肝病的相关性[J]. 临床肝胆病杂志, 2018, 34(10): 2169-2174.

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