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HBV包膜蛋白N-糖基化修饰变异与HBV相关慢加急性肝衰竭发生和转归的关系
Association of N-glycosylation mutation of HBV envelope protein with the development and prognosis of acute-on-chronic liver failure in patients with chronic hepatitis B
文章发布日期:2018年06月07日  来源:  作者:陈杰,姚明琦,魏飞力,等  点击次数:765次  下载次数:63次

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【摘要】:目的 探索HBV包膜蛋白N-糖基化修饰变异与慢性乙型肝炎患者发生慢加急性肝衰竭(ACLF)的关系。方法 入选HBV-ACLF患者90例,同期60例慢性乙型肝炎作对照。套式PCR扩增患者的HBV S 区序列并测序,分析HBV包膜蛋白N-糖基化情况与ACLF发生的关系,并分析ACLF疾病严重程度、90 d生存率与HBV包膜蛋白N-糖基化的关系。结果 慢性乙型肝炎患者纳入分析51例(85.0%),HBV-ACLF患者纳入分析79例(87.8%)。 慢性乙型肝炎患者Asn59 N-糖基化样本33例(64.7%),Asn4 N-糖基化样本21例(41.2%),高于HBV-ACLF患者的Asn59 N-糖基化样本16例(20.3%)(P<0.001)和Asn4 N-糖基化样本14例(17.7%)(P=0.003)。ACLF患者中Asn59、Asn4 N-糖基化与非糖基化患者肝功能水平、HBV DNA、MELD评分及90 d生存率差异均无统计学意义(P值均>0.05)。结论 HBV包膜蛋白变异导致的Asn59、Asn4 N-糖基化水平降低与ACLF发生可能相关。
【Abstract】:Objective To investigate the association of N-glycosylation mutation of HBV envelope protein with the development of acute-on-chronic liver failure (ACLF) in patients with chronic hepatitis B (CHB). Methods A total of 90 patients with HBV-ACLF were enrolled, and 60 patients with CHB were enrolled as control group. Nested PCR was used for the multiplication and sequencing of HBV S region, and the association of N-glycosylation of HBV envelope protein with the development ACLF was analyzed, as well as the association of N-glycosylation of HBV envelope protein with the severity and 90-day survival rate of ACLF. Results A total of 51 CHB patients (85.0%) and 79 HBV-ACLF patients (87.8%) were included in analysis. Among the CHB patients, 33 (64.7%) had Asn-59 N-glycosylation and 21 (41.2%) had Asn-4 N-glycosylation, while among the HBV-ACLF patients, 16 (20.3%) had Asn-59 N-glycosylation and 14 (17.7%) had Asn-4 N-glycosylation; there were significant differences in the numbers of cases of Asn-59 and Asn-4 N-glycosylation between the two groups (P<0.001, and P=0.003). There were no significant differences in liver function, HBV DNA, Model for End-Stage Liver Disease score, and 90-day survival rate between the ACLF patients with Asn-59 and Asn-4 N-glycosylation and those without such glycosylation (P>0.05). Conclusion The reduction in Asn-59 and Asn-4 N-glycosylation induced by HBV envelope protein variation may be associated with the development of ACLF.
【关键字】:肝炎病毒,乙型;肝功能衰竭;病毒包膜蛋白类;糖基化
【Key words】:hepatitis B virus; liver failure; viral envelope proteins; glycosylation
【引证本文】:CHEN J, YAO MQ, WEI FL, et al. Association of N-glycosylation mutation of HBV envelope protein with the development and prognosis of acute-on-chronic liver failure in patients with chronic hepatitis B[J]. J Clin Hepatol, 2018, 34(7): 1428-1431. (in Chinese)
陈杰, 姚明琦, 魏飞力, 等. HBV包膜蛋白N-糖基化修饰变异与HBV相关慢加急性肝衰竭发生和转归的关系[J]. 临床肝胆病杂志, 2018, 34(7): 1428-1431.

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