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洛铂对肝癌HepG2细胞增殖和调亡的作用及机制
Effect of lobaplatin on proliferation and apoptosis of hepatocellular carcinoma HepG2 cells and related mechanism of action
文章发布日期:2018年03月07日  来源:  作者:李莹莹,詹丽芬,曾哲超,等   点击次数:116次  下载次数:9次

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【摘要】:目的在体外细胞中研究洛铂对肝癌HepG2 细胞在增殖、凋亡方面的作用及其机制。方法将HepG2细胞分为洛铂组(增殖实验:5、10、15 μmol/L组;凋亡实验:10 μmol/L;侵袭实验:4 μg/ml)和对照组(不加药物)。利用CCK8法和流式细胞术分别检测2组HepG2 细胞的增殖和凋亡情况。通过Western Blot初步检测2组细胞中增殖、凋亡相关蛋白NF-κB、Bcl-2、Bax、Bid、Puma、Caspase-3的表达变化。增殖实验采用双因素方差分析,凋亡实验采用χ2检验,侵袭实验采用t检验。结果在作用于细胞24、48、72 h后,对照组与实验组细胞生长增殖抑制率差异有统计学意义(F=2735,P<0.01),同一时间不同浓度间、同一浓度不同时间差异均有统计学意义(F分别为1857、1365,P值均<0.01)。2组间细胞凋亡率比较差异有统计学意义(χ2= 1821,P<0001)。实验组HepG2细胞中穿透Transwell 小室的细胞数少于对照组(21.30±2.74 vs 45.00±426,t=11.89,P<0.001)。洛铂组HepG2细胞Bcl-2表达水平下降, NF-κB、Bax、Puma、Caspase-3表达水平升高。结论洛铂可通过影响一系列增殖、凋亡蛋白发挥抑制肝癌细胞增殖及促进凋亡的作用,但细胞内信号通路复杂,需要进一步研究。
【Abstract】:ObjectiveTo investigate the effect of lobaplatin on the proliferation and apoptosis of hepatocellular carcinoma (HCC) HepG2 cells in vitro and related mechanism of action. MethodsHepG2 cells were divided into lobaplatin group (proliferation assay: 5 μmol/L, 10 μmol/L, and 15 μmol/L; apoptosis assay: 10 μmol/L; invasion assay: 4 μg/ml) and control group (no drug was added). CCK-8 assay and flow cytometry were used to measure the proliferation and apoptosis of HepG2 cells. Western Blot was used to measure the change in the expression of NF-κB, Bcl-2, Bax, Bid, Puma, and caspase-3 proteins involved in proliferation and apoptosis. A two-way analysis of variance was used for proliferation assay, the chi-square test was used for apoptosis assay, and the t-test was used for invasion assay. ResultsAfter intervention for 24, 48, and 72 hours, there was a significant difference in proliferation inhibition rate between the two groups (F=273.5, P<0.01); there was also a significant difference between different concentration groups at the same time point (F=1857, P<0.01), and all the concentration groups showed a significant change over time (F=1365, P<0.01). As for apoptosis, there was a significant difference in apoptosis rate between the two groups (χ2=1821, P<0.001). As for invasion ability, compared with the control group, the lobaplatin group had a significantly lower number of cells passing through the Transwell chamber (2130±2.74 vs 45.00±4.26, t=11.89, P<0.001). As for proteins, the lobaplatin group had a significant reduction in the expression of Bcl-2 and significant increases in the expression of NF-κB, Bax, Puma, and caspase-3. ConclusionLobaplatin exerts a certain effect on the proliferation and apoptosis of HCC HepG2 cells by affecting the proteins involved in proliferation and apoptosis, but further studies are needed due to complex signaling pathways in cells.
【关键字】:癌, 肝细胞; 洛铂; 细胞增殖; 细胞凋亡
【Key words】:carcinoma, hepatocellular; lobaplatin; cell proliferation; apoptosis
【引证本文】:LI YY, ZHAN LF, ZENG ZC, et al. Effect of lobaplatin on proliferation and apoptosis of hepatocellular carcinoma HepG2 cells and related mechanism of action[J]. J Clin Hepatol, 2018, 34(4): 784-788. (in Chinese)
李莹莹, 詹丽芬, 曾哲超, 等. 洛铂对肝癌HepG2细胞增殖和调亡的作用及机制[J]. 临床肝胆病杂志, 2018, 34(4): 784-788.

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