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裸鼠皮下泡型棘球蚴病移植瘤模型的建立
Establishment of a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice
文章发布日期:2018年02月07日  来源:  作者:周启锋,张灵强,廖梦娇,等  点击次数:361次  下载次数:46次

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【摘要】:目的建立裸鼠皮下泡型棘球蚴病移植瘤模型,为进一步深入研究泡型棘球蚴病奠定基础。方法选取25只裸鼠进行20%泡型棘球蚴原头节混悬液0.1 ml颈背部皮下注射接种,观察记录病灶生长状况,术后6个月行台盼蓝染色观察原头节细胞活性。结果术后8 d左右可见皮下病灶结节;模型建立3个月后裸鼠存活率为92%(23/25),泡型棘球蚴感染率为100%(23/23);少量原头节死亡,台盼蓝染色蓝色,原头节细胞活力为98%。结论泡型棘球蚴能够成功移植于裸鼠皮下,可为进一步建立泡型棘球蚴病的动物模型提供参考。
【Abstract】:ObjectiveTo establish a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice, and to lay a foundation for further research on alveolar echinococcosis. MethodsA total of 25 nude mice were given subcutaneous injection of 0.1 ml 20% Echinococcus multilocularis protoscolex suspension at the back of the neck. The growth of lesions was observed and recorded, and trypan blue staining was performed at 6 months after injection to observe the cell viability of protoscolices. ResultsSubcutaneous lesions were observed at about 8 days after injection. At 3 months after the establishment of the model, the survival rate of the nude mice was 92% (23/25), and the infection rate of Echinococcus multilocularis was 100% (23/23). A small number of protoscolices died and were blue on trypan blue staining, and the cell viability of protoscolices was 98%. ConclusionEchinococcus multilocularis can be successfully transplanted to the subcutaneous part of nude mice, which provides a reference for further studies on the establishment of animal models of alveolar echinococcosis.
【关键字】:棘球蚴病, 肝; 疾病模型, 动物
【Key words】:echinococcosis, hepatic; disease models, animal
【引证本文】:ZHOU QF, ZHANG LQ, LIAO MJ, et al. Establishment of a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice[J]. J Clin Hepatol, 2018, 34(3): 588-590. (in Chinese)
周启锋, 张灵强, 廖梦娇, 等. 裸鼠皮下泡型棘球蚴病移植瘤模型的建立[J]. 临床肝胆病杂志, 2018, 34(3): 588-590.

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