首 页   本刊简介  编委会  审稿专家  在线期刊  写作规范  广告合作  联系我们
您现在的位置:首页 => 在线期刊 => 6期丙型肝炎 => 肝脏肿瘤 =>microRNA-149-5p过表达对肝..
microRNA-149-5p过表达对肝癌细胞HepG2和Bel-7402增殖及迁移的抑制作用
Inhibitory effect of microRNA-149-5p in the proliferation and migration of HepG2 and Bel-7402 hepatoma cells
文章发布日期:2017年05月04日  来源:  作者:耿亚军,李树栋,刘申政,等  点击次数:299次  下载次数:46次

调整字体大小:

(此处下载失败可以在在线预览处保存副本或者右键另存为)

【摘要】:目的明确microRNA-149-5p(miR-149-5p)在肝癌组织中的表达,并探讨其在肝癌细胞中的分子生物学作用。 方法 收集2010年1月-2014年1月内蒙古医科大学附属医院通过肝切除术获得的65例肝癌组织及相应癌旁组织,采用荧光定量PCR检测65例肝癌组织及相应癌旁组织中miR-149-5p的表达情况。细胞实验分2组,实验组转染miR-149-5p模拟物,对照组转染模拟物阴性对照,采用噻唑蓝比色法和创伤愈合试验检测miR-149-5p对HepG2和Bel-7402细胞增殖及迁移能力的影响。计量资料组间比较采用t检验或单因素方差分析。结果miR-149-5p在肝癌组织的表达量(0.14±0.06)明显低于配对的癌旁组织(2.56±0.42),两者差异有统计学意义(t=7.79,P<005);miR-149-5p在肝癌细胞HepG2、Bel-7402和正常肝上皮细胞L02中的表达量分别为(143±025)、(177±032)和(568±074),3组间比较差异有统计学意义(F=11.27,P<005)。体外功能实验发现,miR-149-5p模拟物可显著抑制HepG2和Bel-7402细胞24、48、72 h时的增殖(HepG2:t值分别为498、5.17、778,P值均<005;Bel-7402:t值分别为683、709、1567,P值均<005),并降低HepG2和Bel-7402细胞的迁移(t值分别为2311、1742,P值均<0.05)。 结论miR-149-5p在肝癌组织中表达下调,过表达miR-149-5p可抑制肝癌细胞的增殖及迁移能力,提示miR-149-5p可作为肝癌基因治疗的一个新的有效的分子靶标。
【Abstract】:ObjectiveTo investigate the expression of microRNA-149-5p (miR-149-5p) in liver cancer tissue and its molecular and biological role in hepatoma cells. MethodsA total of 65 liver cancer tissue samples and corresponding adjacent tissue samples were collected from January 2010 to January 2014, in the Affiliated Hospital of Inner Mongolia Medical University. Quantitative real-time PCR was used to measure the expression of miR-149-5p in liver cancer tissue and corresponding adjacent tissue. The cells were divided into two groups; the cells in the experimental group were transfected with miR-149-5p mimic, and those in the control group were transfected with the negative control of the mimic. MTT colorimetry and wound-healing assay were performed to determine the effect of miR-149-5p on the proliferation and migration of HepG2 and Bel-7402 cells. The t-test or a one-way analysis of variance was used for comparison of continuous data between groups. ResultsThe liver cancer tissue had significantly lower expression of miR-149-5p than the adjacent tissue (0.14±0.06 vs 2.56±0.42, t=7.79, P<0.05). There were significantly differences in the expression of miR-149-5p in HepG2(1.43±0.25)、Bel-7402(1.77±0.32), and the normal hepatic epithelial cells(5.68±0.74)(F=11.27,P<005). The in vitro functional experiment showed that miR-149-5p mimic significantly inhibited the proliferation of 24、48、72 hour of HepG2 and Bel-7402 hepatoma cells (HepG2: t=498,517,778,all P<0.05; Bel-7402:t=683,709,1567,all P<005) and inhibited the migration of HepG2 and Bel-7402 hepatoma cells(t=23.11, 17.42, both P<005). ConclusionThe expression of miR-149-5p is downregulated in liver cancer tissue, and overexpressed miR-149-5p can inhibit the proliferation and migration of hepatoma cells, suggesting that miR-149-5p may be a promising and effective molecular target for the genetic treatment of liver cancer.
【关键字】:肝肿瘤, 实验性; 微RNAs; 细胞增殖; 细胞运动
【Key words】:liver neoplasms, experimental; microRNAs; cell proliferation; cell movement
【引证本文】:耿亚军, 李树栋, 刘申政, 等. microRNA-149-5p过表达对肝癌细胞HepG2和Bel-7402增殖及迁移的抑制作用[J]. 临床肝胆病杂志, 2017, 33(6): 1126-1130.

地址:长春市东民主大街519号《临床肝胆病杂志》编辑部 邮编:130061 电话:0431-88782542/3542
临床肝胆病杂志 版权所有 Copyright © 2009 - 2013 Lcgdbzz.org. All Rights Reserv 吉ICP备10000617号

吉公网安备 22010402000041号