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蓝莓联合益生菌对非酒精性脂肪性肝病模型大鼠PNPLA3和SREBP-1c表达的影响
Effect of blueberry and probiotics on expression of PNPLA3 and SREBP-1c in rats with nonalcoholic fatty liver disease
文章发布日期:2016年08月08日  来源:  作者:祝娟娟, 周明玉, 程明亮  点击次数:1127次  下载次数:220次

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【摘要】:目的观察含patatin样磷脂酶域3(PNPLA3)和胆固醇调节元件结合蛋白(SREBP)1c在蓝莓联合益生菌对非酒精性脂肪性肝病(NAFLD)模型大鼠干预实验中的表达,并探讨其在NAFLD中的作用机制。方法清洁级SD大鼠36只分为正常组(NG)、模型组(MG)、自然恢复组(SRG)、蓝莓组(BG)、益生菌组(PG)和蓝莓联合益生菌组(BPG)。实验结束后,制作组织切片,观察血清学、组织学形态变化,检测PNPLA3和SREBP-1c基因及蛋白表达情况。多组间比较用单因素方差分析,方差齐时用SNK-q检验,方差不齐时用Tamhane′s-T2检验。结果6组间在肝脏指数、ALT、TC、TG、LDL和HDL上差异均有统计学意义(F值分别为384908、188.554、75.523、94.667、95.235、132.586,P值均<0.01)。6组间NAFLD活动度积分差异有统计学意义(F=71896,P<0.01),其中BPG组较MG、SRG、BG、PG组下降最为显著(P值均<001)。6组肝组织表达PNPLA3和SREBP-1c阳性率差异均有统计学意义(F值分别为175.527、110.504,P值均<0.01),其中BPG组较MG、SRG、BG、PG组下降最为显著(P值均<001)。PNPLA3和SREBP-1c的mRNA和蛋白表达各组间差异有统计学意义(F值分别为375822、410379、288940、116054,P值均<001)。其中BPG组的PNPLA3和SREBP-1C的mRNA和蛋白表达较MG、SRG、BG、PG组明显下降(P值均<001)。结论蓝莓联合益生菌能有效改善NAFLD的病理组织结构,减轻肝细胞脂肪变性,其机制可能是蓝莓与益生菌联合可减轻炎症因子诱发的炎症效应,下调SREBP-1c的表达,从而减弱PNPLA3基因转录,增强胆固醇代谢,减少脂质沉积,是NAFLD的一个辅助治疗方案。
【Abstract】:ObjectiveTo investigate the expression of patatin-like phospholipase domain-containing protein 3(PNPLA3) and sterol regulatory element-binding protein-1c (SREBP-1c) in the rat model of nonalcoholic fatty liver disease (NAFLD) treated by blueberry combined with probiotics, as well as its mechanism of action in NAFLD. MethodsA total of 36 clean Sprague-Dawley rats were divided into normal group (NG), model group (MG), spontaneous recovery group (SRG), blueberry group (BG), probiotics group (PG), and blueberry-probiotics group (BPG). Tissue sections were prepared after the experiment ended to observe serological and histological changes and measure the mRNA and protein expression of PNPLA3 and SREBP-1c. A one-way analysis of variance was used for comparison between multiple groups; the SNK-q test was used for data with homogeneity of variance, and the Tamhane′s-T2 test was used for data with heterogeneity of variance. ResultsThe six groups showed significant differences in liver index, serum alanine aminotransferase, total cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein (F=384.908, 188.554, 75.523, 94.667, 95.235, and 132586, all P<0.01). The NAFLD activity score also showed a significant difference between the six groups (F=71.896, P<0.01), and BPG showed a significantly greater reduction in the NAFLD activity score than MG, SRG, BG, and PG (all P<001). The six groups showed significant differences in the positive rates of PNPLA3 and SREBP-1c in liver tissue (F=175.527 and 110.504, both P<0.01), and BPG showed a significantly greater reduction in the positive rates of PNPLA3 and SREBP-1C than MG, SRG, BG, and PG (all P<001). The mRNA and protein expression of PNPLA3 and SREBP-1c showed a significantly difference between the six groups. (F=375822, 410379, 288940, 116054, all P<001). BPG showed significant reductions in the mRNA expression of PNPLA3 and SREBP-1c compared with MG, SRG, BG, and PG (all P<0.01). ConclusionBlueberry combined with probiotics can effectively improve the structure of pathological tissues in NAFLD and reduce hepatocyte fatty degeneration. Its mechanism may be that the combination of blueberry and probiotics can reduce inflammation induced by inflammatory factors and downregulate the expression of SREBP-1c, and thus weaken PNPLA3 gene transcription, enhance cholesterol metabolism, and reduce lipid deposition. Therefore, it can be applied as adjuvant therapy for NAFLD.
【关键字】:脂肪肝; 含patatin样磷脂酶域3; 胆固醇调节元件结合蛋白质1; 大鼠, Sprague-Dawley
【Key words】:fatty liver; patatin-like phospholipase clomain containing 3; sterol regulatory element binding protein 1; rats, Sprague-Dawley
【引证本文】:祝娟娟, 周明玉, 程明亮. 蓝莓联合益生菌对非酒精性脂肪性肝病模型大鼠PNPLA3和SREBP-1c表达的影响[J]. 临床肝胆病杂志, 2016, 32(9): 1778-1784.

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