首 页   本刊简介  编委会  审稿专家  在线期刊  写作规范  广告合作  联系我们
您现在的位置:首页 => 在线期刊 => 2012年 12期 => 病毒性肝炎 =>吉林省白细胞介素-2基因+114..
吉林省白细胞介素-2基因+114 (G/T )位点多态性与慢性乙型肝炎、肝硬化和肝癌易感性的相关性分析
Association between IL-2 +114 (G/T) polymorphism and susceptibility to hepatitis B, cirrhosis, and hepatocellular carcinoma in Jilin province
文章发布日期:2012年11月12日  来源:  作者:洪玉波 张婷 孙丽光  点击次数:2186次  下载次数:426次

调整字体大小:

(此处下载失败可以在在线预览处保存副本或者右键另存为)

【摘要】:目的研究白细胞介素-2(IL-2)基因+114 (G/T)位点多态性在吉林省健康人群与乙型肝炎、肝硬化及肝癌患者中的分布及频率;探讨IL-2基因的单核苷酸多态性(SNP)与乙型肝炎、肝硬化及肝癌之间的关系。方法应用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)等技术检测吉林省慢性乙型肝炎 (CHB) 患者115例、乙型肝炎背景下的肝硬化(LC)患者78例和肝癌(HCC)患者115例及健康体检者107例的IL-2基因+114位点的多态性,并用DNA测序法进行验证,对四组中基因型及等位基因的分布进行统计学分析。结果IL-2基因+114 (G/T)位点共有三种基因型:GG型、TG型和TT型。各基因型在病例组与对照组之间差异无统计学意义(P>0.05)。CHB组、LC组中T等位基因所占比例与对照组相比差异具有统计学意义(P=0011,P=0.019),HCC组与对照组的差异无统计学意义(P>0.05)。提示T等位基因与CHB、HCC的患病风险有关(OR=0.612,P=0.011;OR=0562,P=0.019)。结论IL-2基因+114(G/T)多态性在吉林省健康体检者与CHB、LC及HCC患者中的分布具有显著性差异,等位基因T分布频率与CHB及LC的患病风险呈负相关性。
【Abstract】:Abstract:ObjectiveTo research the distributions and frequencies of +114 (G/T) polymorphism of the interleukin-2 (IL-2) gene among the population from Jilin province and to investigate the association with susceptibility to chronic hepatitis B (CHB). MethodsPolymerase chain reaction-restriction fragment length polymorphism was used to detect the IL-2 +114 G/T polymorphism in 115 CHB patients, 78 hepatitis B virus (HBV)-related liver cirrhosis (LC) patients, 115 HBV-associated hepatocellular carcinoma (HCC) patients, and 107 healthy controls. The significance of intergroup differences was assessed by the Chi-squared test. ResultsThree genotypes of IL-2 +114 (G/T) were detected: TT, TG and GG. However, the frequency distributions between the groups did not reach statistical significance (all P>0.05). Unlike the G allele, the T allele was associated with susceptibility to CHB (P=0.011) and LC (P=0.019); in addition, the T allele was associated with a markedly lower risk of CHB [odds ratio (OR)=0.612] and LC (OR=0.562). ConclusionThe IL-2 +114 (G/T) polymorphism is associated with susceptibility to CHB and LC in Chinese from the Jilin province. Specifically, the T allele is negatively correlated to risk of CHB and LC.
【关键字】:肝炎,乙型;肝硬化;白细胞介素2;多态性,单核甘酸
【Key words】:hepatitis B; liver cirrhosis; interleukin-2; polymorphism, single nucleotide
【引证本文】:

地址:长春市东民主大街519号《临床肝胆病杂志》编辑部 邮编:130061 电话:0431-88782542/3542
临床肝胆病杂志 版权所有 Copyright © 2009 - 2013 Lcgdbzz.org. All Rights Reserv 吉ICP备10000617号

吉公网安备 22010402000041号