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肝癌和肝硬化患者庚型肝炎病毒RNA及NS3区cDNA酶切分析
Detection of hepatitis G virus RNA and restrict endonulease analysis of NS3 gene cDNA in sera of patients with hepatocellular carcinoma and liver cirrhosis
文章发布日期:2009年10月26日  来源:  作者:杜绍财; 程恳; 常锦红  点击次数:1767次  下载次数:614次

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【摘要】:目的探讨肝癌及肝硬化患者中HGV的感染状态及NS3区部分基因的酶切分析。采用RTPCR技术检测HGVRNA,酶切分析比较中国株与美国GBV-C株两种基因型的酶切位点。结果81例肝癌样品中9例HGVRNA阳性(11.11%),28例肝硬化中,3例HGVRNA阳性(10.7%)。酶切位点分析结果表明,中国HGV感染患者在GBV-C4351位存在TSP509I(AA↓TT)切点,美国和日本发表的11株GBV-C和1株HGV中仅2株存在此切点。结论酶切分析结果提示GBV-C株与中国HGV感染株,可能不属于同一基因型
【Abstract】:To detect the presence of hepatitis G virus infection in patients with hepatocellular carcinoma and liver cirrhosis and analyze the NS3 gene with restrict endounclease.RT PCr technique was used to detect HGV RNA.Positive PCR products were digested with restrict endonuclease to find the existence of certain recognization site.Results:9 samples(11.1%) were HGV RNA positive in 81 patints with hepatocellular carcinoma,and 3(10.7%) were positive in 28 patients with liver cirrhosis.When digested with restrict endonuclease,all Chinese isolates had TSP 509I (AA↓TT) recognization site in nt 4351 (GBVC position),however,only 2 of 12 American and Japanese isolates (11 GBVC isolates and 1 HGV isolate)had this site.Conclusion:The result of restrict endonuclease analysis demonstrated that Chinese HGv isolate and GBVC isolate may belong to different genotype.
【关键字】:庚型肝炎病毒RNA; 聚合酶链式反应; 基因型; 肝癌
【Key words】:hepatitis G virus polymerase chain reaction genotype hepatocellular carcinomaliver cirrhosis
【引证本文】:

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