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环氧合酶2抑制剂对非酒精性脂肪性肝病大鼠模型回肠Acsl基因家族表达的影响
Effect of cyclooxygenase-2 inhibitors on the expression of the Acsl gene family in the ileum of rats with nonalcoholic fatty liver disease
文章发布日期:2020年09月25日  来源:  作者:郭珊,易世杰,阳学风,等  点击次数:578次  下载次数:38次

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【摘要】:目的 研究环氧合酶2(COX-2)抑制剂对非酒精性脂肪性肝病(NAFLD)大鼠模型回肠Acsl基因家族表达的影响及意义。方法 将45只SD大鼠随机分为3组:正常对照组(n=15只,普通饲料)、NAFLD模型组(n=15,高脂饲料)、尼美舒利组(n=15,高脂饲料+尼美舒利)。所有SD大鼠喂养到第12周处死。采集下腔静脉血,检测其总胆固醇(TC)、甘油三酯(TG)。肝组织行HE染色和油红O染色,评价各组大鼠肝组织脂肪变性程度。实时荧光定量PCR法检测回肠Acsl家族各基因的表达情况。计量资料多组间比较采用方差分析,进一步两两比较采用LSD-t检验。结果 与正常对照组大鼠比较,NAFLD模型组血清TC、TG值均明显增加,肝脏脂肪变性明显(P值均<0.05);尼美舒利组与NAFLD模型组比较,血清TC、TG值均明显下降,肝脏脂肪变性程度减轻(P值均<0.05);与正常对照组大鼠相比,NAFLD模型组大鼠回肠COX-2的表达明显升高(P<0.05);与NAFLD模型组大鼠相比,尼美舒利组回肠COX-2的表达明显降低(P<0.05);与正常对照组相比,NAFLD模型组回肠Acsl3、Acsl5基因的表达明显增加(P值均<0.05);与NAFLD模型组相比,尼美舒利组Acsl3、Acsl5基因的表达明显降低(P值均<0.05)。结论 COX-2抑制剂尼美舒利对NAFLD大鼠回肠Acsl基因家族表达有调控作用,提示COX-2抑制剂可能通过Acsl基因抑制NAFLD的进展。
【Abstract】:Objective To investigate the effect and significance of cyclooxygenase-2 (COX-2) inhibitors on the expression of the Acsl gene family in the ileum of rats with nonalcoholic fatty liver disease (NAFLD). Methods A total of 45 Sprague-Dawley rats were randomly divided into normal control group (15 rats given normal diet), NAFLD model group (15 rats given high-fat diet), and nimesulide group (15 rats given high-fat diet and nimesulide). All rats were sacrificed after 12 weeks of feeding, and then blood samples were collected from the inferior vena cava to measure total cholesterol (TC) and triglyceride (TG). HE staining and oil red O staining were performed for the liver to evaluate the degree of hepatic steatosis in each group, and quantitative real-time PCR was used to measure the mRNA expression of the Acsl family genes in the ileum. An analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. Results Compared with the normal control group, the NAFLD model group had significant increases in serum TC and TG and marked hepatic steatosis (all P<0.05); compared with the NAFLD model group, the nimesulide group had significant reductions in serum TC and TG and degree of hepatic steatosis (all P<0.05). Compared with the normal control group, the NAFLD model group had a significant increase in the expression of COX-2 in the ileum (P<0.05), and compared with the NAFLD model group, the nimesulide group had a significant reduction in the expression of COX-2 in the ileum (P<0.05). Compared with the normal control group, the NAFLD model group had significant increases in the mRNA expression of Acsl3 and Acsl5 in the ileum (both P<0.05), and compared with the NAFLD model group, the nimesulide group had significant reductions in the mRNA expression of Acsl3 and Acsl5 (both P<0.05). Conclusion The COX-2 inhibitor nimesulide can regulate the expression of the Acsl gene family in the ileum of rats with NAFLD, suggesting that COX-2 inhibitors may inhibit the progression of NAFLD through the Acsl gene.
【关键字】:环氧化酶2; 非酒精性脂肪性肝病; 大鼠,Sprague-Dawley; 基因
【Key words】:cyclooxygenase 2; non-alcoholic fatty liver disease; rats,sprague-dawley; genes
【引证本文】:GUO S, YI SJ, YANG XF, et al. Effect of cyclooxygenase-2 inhibitors on the expression of the Acsl gene family in the ileum of rats with nonalcoholic fatty liver disease[J]. J Clin Hepatol, 2020, 36(9): 2040-2044. (in Chinese)
郭珊, 易世杰, 阳学风, 等. 环氧合酶2抑制剂对非酒精性脂肪性肝病大鼠模型回肠Acsl基因家族表达的影响[J]. 临床肝胆病杂志, 2020, 36(9): 2040-2044.

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