首 页   本刊简介  编委会  审稿专家  在线期刊  写作规范  广告合作  联系我们
您现在的位置:首页 => 在线期刊 => 2020年 7期“炎症性肠病与肝胆胰疾病” => 其他肝病 =>肝爽颗粒对新型组织工..
肝爽颗粒对新型组织工程肝脏非酒精性脂肪性肝病大鼠模型脂代谢的影响
Effect of Ganshuang granules on lipid metabolism in a new tissue-engineering model of nonalcoholic fatty liver disease
文章发布日期:2020年06月08日  来源:  作者:张译之,段钟平,张晓慧,等  点击次数:324次  下载次数:39次

调整字体大小:

(此处下载失败可以在在线预览处保存副本或者右键另存为)

【摘要】:目的 探讨肝爽颗粒(GSG)干预对新型组织工程肝脏非酒精性脂肪性肝病(NAFLD)模型脂代谢的影响及其机制。方法 将Sprague Dawley大鼠肝脏去细胞化制为肝脏胶原支架,用人HepG2细胞对支架再细胞化,从而获得组织工程(TE)肝(正常对照组),用含游离脂肪酸(FFA)的高脂培养基灌注TE肝建立NAFLD模型(FFA组),该模型进一步用GSG浸膏处理后为FFA+GSG组。比较各组肝脏TG含量及丙酮酸脱氢酶激酶4(PDK4) mRNA水平;比较FFA组和FFA+GSG组脂代谢相关酶mRNA表达情况,并行油红O染色评估肝脏病理学。计量资料组间差异比较采用t检验。结果 FFA组TG含量较正常对照组显著升高(t=4.842,P=0.004 7),FFA组PDK4 mRNA水平较正常组显著升高(t=2.784,P=0.031 8);FFA+GSG组较FFA组细胞内TG水平显著下降(t=0.055,P=0.003 7),PDK4表达显著降低(t=3.761,P=0.009 4);脂肪酸转位酶、脂肪酸结合蛋白1、ATP柠檬酸裂解酶、乙酰辅酶A羧化酶、脂肪酸合成酶、脂肪酸去饱和酶2、1-酰基甘油-3-磷酸O-酰基转移酶5及载脂蛋白B mRNA表达水平显著降低(t值分别为6.552、4.944、2.689、4.524、6.040、3.758、4.443、3.032,P值分别为0.007 2、0.001 1、0.027 6、0.020 2、0.009 1、0.005 6、0.047 1和0.016 3);油红O染色显示肝细胞内脂滴显著减少,肝细胞脂肪变性程度明显减轻。结论 GSG可减少FFA摄取、降低脂肪酸从头合成,减少NAFLD模型肝脏胞内TG的生成和沉积,改善脂代谢紊乱。
【Abstract】:Objective To investigate the effect of Ganshuang granules (GSG) intervention on lipid metabolism in a new tissue-engineering model of nonalcoholic fatty liver disease (NAFLD) and its mechanism. Methods The liver of Sprague-Dawley rats was decellularized into collagen scaffolds, and human HepG2 cells were used to recellularize the scaffolds to obtain the tissue-engineering (TE) liver as normal control group. The TE liver was perfused with high-fat medium containing free fatty acid (FFA) to establish a model of NAFLD (FFA group), and this model was further treated with GSG extract to establish a FFA+GSG group. These groups were compared in terms of the content of triglyceride (TG) and the mRNA expression of PDK4 in the liver, and the mRNA expression of enzymes associated with lipid metabolism was compared between the FFA group and the FFA+GSG group; oil red O staining was used to evaluate liver pathology. The t-test was used for comparison of continuous data between groups. Results Compared with the normal control group, the FFA group had significantly higher content of TG (t=4.842, P=0.004 7) and mRNA expression of PDK4 (t=2.784, P=0.031 8). Compared with the FFA group, the FFA+GSG group had significant reductions in the content of TG in cells (t=0.055, P=0.003 7), the expression of PDK4 (t=3.761, P=0.009 4), and the mRNA expression of fatty acid translocase, fatty acid-binding protein 1, ATP citrate lyase, acetyl-CoA carboxylase, fatty acid synthase, fatty acid desaturase 2, 1-acylglycerol-3-phosphate O-acyltransferase 5, and apolipoprotein B (t=6.552, 4.944, 2.689, 4.524, 6.040, 3.758, 4.443, and 3.032, P=0.007 2, 0.001 1, 0.027 6, 0.020 2, 0.009 1, 0.005 6, 0.047 1, and 0.016 3). Oil red O staining showed significant reductions in the number of lipid droplets in hepatocytes and the degree of hepatocyte fatty degeneration. Conclusion GSG can reduce the intake of FFA, the de novo synthesis of fatty acid, and the generation and deposition of TG in hepatocytes in the model of NAFLD and thus improve dyslipidemia.
【关键字】:非酒精性脂肪性肝病; 组织工程; 肝爽颗粒; 大鼠,Sprague-Dawley
【Key words】:nonalcoholic fatty liver disease; tissue engineering; Ganshuang granule; rats,Sprague-Dawley
【引证本文】:ZHANG YZ, DUAN ZP, ZHANG XH, et al. Effect of Ganshuang granules on lipid metabolism in a new tissue-engineering model of nonalcoholic fatty liver disease[J]. J Clin Hepatol, 2020, 36(7): 1551-1555. (in Chinese)
张译之, 段钟平, 张晓慧, 等. 肝爽颗粒对新型组织工程肝脏非酒精性脂肪性肝病大鼠模型脂代谢的影响[J]. 临床肝胆病杂志, 2020, 36(7): 1551-1555.

地址:长春市东民主大街519号《临床肝胆病杂志》编辑部 邮编:130061 电话:0431-88782542/3542
临床肝胆病杂志 版权所有 Copyright © 2009 - 2013 Lcgdbzz.org. All Rights Reserv 吉ICP备10000617号

吉公网安备 22010402000041号