首 页   本刊简介  编委会  审稿专家  在线期刊  写作规范  广告合作  联系我们
您现在的位置:首页 => 在线期刊 => 2020年 6期“非酒精性脂肪性肝病与多代谢紊乱的基础与临床” => 肝脏肿瘤 => 细胞外泛素对肝癌细..
细胞外泛素对肝癌细胞增殖、侵袭和迁移能力的影响
Effect of extracellular ubiquitin on the proliferation, invasion, and migration of hepatoma cells
文章发布日期:2020年06月01日  来源:  作者:张杨,陈淑英,徐光如  点击次数:152次  下载次数:29次

调整字体大小:

(此处下载失败可以在在线预览处保存副本或者右键另存为)

【摘要】:目的 通过体外细胞实验,探讨细胞外泛素对肝细胞癌细胞增殖、侵袭和迁移能力的影响。方法 利用不同浓度的细胞外泛素(200、400、800 ng/ml),分别干预肝癌细胞(HepG2),采用CCK8法检测细胞的增殖情况;利用Transwell法检测不同浓度泛素对肝癌细胞侵袭能力的影响;利用划痕实验和蛋白免疫印迹检测细胞外泛素对肝癌细胞迁移能力的作用。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果 CCK8法检测结果显示,细胞外泛素干预后能明显促进肝癌细胞的增殖能力,并具有明显的浓度依赖性,且泛素干预浓度在400 ng/ml时作用最显著,在48、72、96 h相对吸光度值均明显高于对照组(P值均<0.05)。Transwell实验结果显示,与对照组相比,不同浓度的细胞外泛素均可明显促进肝癌细胞的侵袭,且在400 ng/ml泛素干预组最显著[(134.00 ± 8.18)个 vs (347.33±18.90)个,P<0.001]。划痕实验和蛋白免疫印迹结果显示,400 ng/ml泛素能显著增加HepG2肝癌细胞的迁移能力。结论 细胞外泛素在体外能明显促进HepG2肝癌细胞的增殖、侵袭和迁移,且具有浓度依赖性。
【Abstract】:Objective To investigate the effect of extracellular ubiquitin on the proliferation, invasion, and migration of hepatoma cells by in vitro cell experiments. Methods The hepatoma cells (HepG2) were treated with extracellular ubiquitin at different concentrations (200, 400, and 800 ng/ml); CCK-8 assay was used to observe cell proliferation, Transwell assay was used to observe the effect of extracellular ubiquitin at different concentrations on the invasion ability of hepatoma cells, and wound healing assay and Western blotting were used to observe the effect of extracellular ubiquitin on the migration ability of hepatoma cells. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. ResultsThe CCK-8 assay showed that extracellular ubiquitin significantly promoted the proliferation of hepatoma cells in a concentration-dependent manner, with the most significant effect at the concentration of 400 ng/ml, and the intervention group had a significantly higher relative absorbance value than the control group at 48, 72, and 96 hours (all P<0.05). The Transwell assay showed that different concentrations of extracellular ubiquitin significantly promoted the invasion of hepatoma cells, with the most significant effect in the group treated by 400 ng/ml extracellular ubiquitin (134.00±8.18 vs 347.33±18.90, P<0.001). The Wound healing assay and Western blotting showed that 400 ng/ml extracellular ubiquitin significantly increased the migration ability of HepG2 cells. Conclusion Extracellular ubiquitin can significantly promote the proliferation, invasion, and migration of HepG2 cells in vitro in a concentration-dependent manner.
【关键字】:癌,肝细胞; 泛素; 细胞增殖; 肿瘤侵润; 细胞运动
【Key words】:carcinoma,hepatocellular; ubiquitin; cell proliferation; neoplasm invasiveness; cell movement
【引证本文】:ZHANG Y, CHEN SY, XU GR. Effect of extracellular ubiquitin on the proliferation, invasion, and migration of hepatoma cells[J]. J Clin Hepatol, 2020, 36(6): 1289-1292. (in Chinese)
张杨, 陈淑英, 徐光如. 细胞外泛素对肝癌细胞增殖、侵袭和迁移能力的影响[J]. 临床肝胆病杂志, 2020, 36(6): 1289-1292.

地址:长春市东民主大街519号《临床肝胆病杂志》编辑部 邮编:130061 电话:0431-88782542/3542
临床肝胆病杂志 版权所有 Copyright © 2009 - 2013 Lcgdbzz.org. All Rights Reserv 吉ICP备10000617号

吉公网安备 22010402000041号