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干扰素α和胸腺五肽协同干预对HepG2.2.15细胞APOBEC3A和APOBEC3B mRNA表达的影响
Effect of interferon-α and thymopentin on the mRNA expression of APOBEC3A and APOBEC3B in HepG2.2.15 cells
文章发布日期:2019年12月20日  来源:  作者:熊芳,高耀,马艳品,等  点击次数:412次  下载次数:109次

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【摘要】:目的 探讨IFNα和胸腺五肽(TP5)协同干预对HepG2.2.15细胞载脂蛋白B mRNA编辑酶催化多肽3A(APOBEC3A)、APOBEC3B基因mRNA表达水平的影响。方法 将HepG2.2.15细胞分为空白对照组、IFN处理组、TP5处理组和IFNα联合TP5组,在处理的12、24、48和72 h用实时荧光定量PCR方法检测细胞APOBEC3A、APOBEC3B基因mRNA表达水平。计量资料多组间比较采用方差分析,进一步两两比较采用LSD-t检验。结果 与空白对照组相比,IFNα、IFNα联合TP5分别处理12、24、48和72 h后,APOBEC3A mRNA的表达水平显著提高,差异均有统计学意义(P值均<0.001)。与IFNα处理组相比,4个时间点IFNα联合TP5组APOBEC3A mRNA的表达水平显著提高,差异均有统计学意义(P值<0.001)。TP5处理在各时间点对APOBEC3A mRNA表达水平的影响差异无统计学意义(P值均>0.05)。与对照组相比,所有处理组中APOBEC3B的mRNA表达水平差异无统计学意义(P值均>0.05)。结论 IFNα联合TP5处理可显著上调HepG2.2.15细胞中APOBEC3A mRNA的表达水平。
【Abstract】:Objective To investigate the effect of synergistic intervention of interferonα (IFNα) and thymopentin (TP5) on the mRNA expression of apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A (APOBEC3A) and apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B (APOBEC3B) in HepG2.2.15 cells. Methods HepG2.2.15 cells were divided into blank control group, IFNα treatment group, TP5 treatment group, and IFNα+TP5 treatment group, and at 12, 24, 48, and 72 hours of treatment, quantitative real-time PCR was used to measure the mRNA expression of APOBEC3A and APOBEC3B in HepG2.2.15 cells. An analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. Results Compared with the blank control group, the IFNα treatment group and the IFNα+TP5 treatment group had a significant increase in the mRNA expression of APOBEC3A at 12, 24, 48, and 72 hours of treatment (all P<0.001). Compared with the IFNα treatment group, the IFNα+TP5 treatment group had a significant increase in the mRNA expression of APOBEC3A at these four time points (all P<0.001). TP5 treatment had no significant influence on the mRNA expression of APOBEC3A at each time point (all P>0.05). There was no significant difference in the mRNA expression of APOBEC3B between the blank control group and the treatment groups (all P>0.05). Conclusion IFNα combined with TP5 can significantly upregulate the mRNA expression of APOBEC3A in HepG2.2.15 cells.
【关键字】:干扰素α; 胸腺喷丁; APOBEC脱氨酶类
【Key words】:interferon-alpha; thymopentin; APOBEC deaminases
【引证本文】:XIONG F, GAO Y, MA YP, et al. Effect of interferon-α and thymopentin on the mRNA expression of APOBEC3A and APOBEC3B in HepG2.2.15 cells[J]. J Clin Hepatol, 2020, 36(1): 76-79. (in Chinese)
熊芳, 高耀, 马艳品, 等. 干扰素α和胸腺五肽协同干预对HepG2.2.15细胞APOBEC3A和APOBEC3B mRNA表达的影响[J]. 临床肝胆病杂志, 2020, 36(1): 76-79.

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