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基于癌症基因组图集数据库分析锌指蛋白580 mRNA在肝细胞癌中的表达及意义
Expression and clinical significance of ZNF580 mRNA in hepatocellular carcinoma: An analysis based on The Cancer Genome Atlas database
文章发布日期:2019年09月05日  来源:  作者:毛 骏,李仁礼,李 海,等  点击次数:126次  下载次数:17次

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【摘要】: 目的 研究锌指蛋白580(ZNF580)mRNA在肝细胞癌中的表达以及临床价值,预测ZNF580 mRNA在肝细胞癌发生发展中的作用。方法 从癌症基因组图集(TCGA)数据库下载肝细胞癌数据集,获得ZNF580 mRNA基因表达谱和临床信息。利用相关生物信息学方法,分析ZNF580 mRNA在肝细胞癌中表达的情况与临床病理指标的相关性以及对预后的影响。用基因富集化分析预测ZNF580 mRNA在肝细胞癌中调控的可能通路。计量资料2组间比较采用t检验,计数资料组间比较采用χ2检验,并运用Cox比例风险回归模型分析影响患者预后的危险因素,计算风险比(HR)及95%可信区间(95%CI)。结果 在TCGA数据库中,ZNF580 mRNA的表达水平在肝癌组织中明显高于癌旁组织(8.440±0.705 vs 7.736±0.387,P<0.05),其表达水平在不同肿瘤分级的患者中差异有统计学意义(t=-2.068,P<0.05)。ZNF580 mRNA高表达患者的总体生存期明显低于ZNF580 mRNA低表达患者(χ2=5.456,P<0.05)。多因素Cox回归分析提示ZNF580 mRNA的表达水平[HR(95%CI):1.600(1.079~2.372)]和TNM分期[HR(95%CI):2.006(1.394~2.888)]是影响肝细胞癌患者总体生存期的独立危险因素(P值均<0.05)。ZNF580 mRNA高表达的样本存在核糖体、亨廷顿病两个通路基因集的富集(P值均<0.05,错误发现率分别为0.198、0.243)。结论 ZNF580 mRNA可能作为促癌基因在肝细胞癌中发挥作用,有望成为判断肝细胞癌预后的指标和潜在的新治疗靶点。
【Abstract】: Objective To investigate the expression and clinical value of ZNF580 mRNA in hepatocellular carcinoma (HCC), as well as the role of ZNF580 mRNA in the development and progression of HCC. Methods HCC dataset was downloaded from The Cancer Genome Atlas (TCGA) to obtain the gene expression profile and clinical information of ZNF580 mRNA. The bioinformatics method was used to analyze the association between the expression of ZNF580 mRNA in HCC and clinicopathological indices and the influence of ZNF580 mRNA on prognosis. A gene enrichment analysis was used to predict the possible pathways of ZNF580 mRNA involved in the regulation of HCC. The t-test was used for comparison of continuous data between two groups, the chi-square test was used for comparison of categorical data between two groups. The Cox proportional-hazards regression model was used to investigate the risk factors for prognosis, and hazard ratio (HR) and 95% confidence interval (CI) were calculated. Results In the TCGA dataset, the expression of ZNF580 mRNA in tumor tissue was significantly higher than that in adjacent tissue (8.440±0.705 vs 7.736±0.387, P<0.05), and there was a significant difference in the expression of ZNF580 mRNA between the patients with different tumor grades (t=-2.068, P<0.05). The patients with high expression of ZNF580 mRNA had a significantly shorter overall survival time than those with low expression (χ2=5.456, P<0.05). The multivariate Cox regression analysis showed that ZNF580 mRNA expression [HR(95%CI)=1.600(1.079~2.372)] and TNM stage [HR(95%CI)=2.006(1.394~2.888)] were independent risk factors for overall survival time of HCC patients (both P<0.05). The samples with high expression of ZNF580 mRNA showed enrichment of genes in the pathways of ribosome and Huntington’s disease (all P<0.05, FDR=0.198,0.243). Conclusion ZNF580 mRNA may act as an oncogene in HCC and is expected to become an indicator for prognostic evaluation of HCC and a potential therapeutic target for HCC.
【关键字】:癌, 肝细胞; 锌指蛋白580 mRNA; 计算生物学; 危险因素
【Key words】:hepatocellular carcinoma; ZNF580 mRNA; computational biology; risk factors
【引证本文】:MAO J, LI RL, LI H, et al. Expression and clinical significance of ZNF580 mRNA in hepatocellular carcinoma: An analysis based on The Cancer Genome Atlas database[J]. J Clin Hepatol, 2019, 35(10): 2231-2235. (in Chinese)
毛骏, 李仁礼, 李海, 等. 基于癌症基因组图集数据库分析锌指蛋白580 mRNA在肝细胞癌中的表达及意义[J]. 临床肝胆病杂志, 2019, 35(10): 2231-2235.

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