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实验性肝纤维化大鼠肝组织中纤溶酶原激活物抑制剂-1分布与表达
Study on the expression and location of PAI-1 in the experimental liver fibrosis rats'livers
文章发布日期:2009年10月10日  来源:  作者:  点击次数:1460次  下载次数:664次

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【摘要】: 为动态观察肝纤维化大鼠模型肝组织中PAI-1的分布和表达,采用CC l4制备肝纤维化大鼠模型进行观察试验。通过对模型肝组织进行PAI-1免疫组化染色和采用图文分析系统检测PAI-1面密度。结果如下:PAI-1阳性染色主要分布在肝纤维化活跃的汇管区、肝组织变性坏死处;PAI-1面密度随注射CC l4时间的延长而递增(3周0.1356±1.052E-2;6周0.1979±1.191E-2;9周0.2321±2.159E-2,P<0.05),与α-SMA阳性细胞数、胶原面积、HPY含量变化一致;停止注射四氯化碳3周时PAI-1面密度明显下降(7.966E-2±5.587E-3)低于6周时(P<0.05),与α-SMA阳性细胞数变化一致,而胶原面积、HPY含量与9周时相同,无明显下降。肝组织中PAI-1的表达与星状细胞活化有关,可反映肝纤维化的进展,但不能反映肝纤维化程度。
【Abstract】:To observe dynamically the expression and location of PAI-1 in the liver of liver fibrosis rats.Liver fibrosis model rats were made by CCl4.The immunohistochemistry staining of PAI-1 in the liver sample and the areadensity of PAI-1 was performed.PAI-1 located mostly the fibrosis portal area,denatured and necrotic liver tissue.Before 9 weeks,PAI-1 went up gradually with time(3 weeds 0.1356±1.052E-2,6 weeds 0.1979±1.191E-2,9 weeds 0.2321±2.159E-2,P<0.05).The level of α-SMA,the area of collagen and the homogenate HPY consisted with PAI-1.At the 12th week,the CCl4 treatment having stopped for 3 weeks,the level of PAI-1(7.966E-2±5.587E-3)and α-SMA was lower than it at the 6th week.But compared with 9th week,the area of collagen and the homogenate HPY didn't decrease.The expression of PAI-1 in liver tissue relates with activity HSCs. It reflects the development of liver fibrosis but not the degree.
【关键字】:纤溶酶原激活物抑制剂-1; 肝纤维化; 大鼠模型
【Key words】:plasminogen activator inhibitor type 1; liver fibrosis; model rat
【引证本文】:

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