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慢性HBV感染者外周血ATF6水平检测及其临床意义
Measurement and clinical significance of activating transcription factor 6 level in peripheral blood in patients with chronic HBV infection
文章发布日期:2019年08月19日  来源:  作者:潘高峰,李国云,季雪良,等  点击次数:108次  下载次数:17次

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【摘要】:目的了解慢性HBV感染者外周血活化转录因子6(ATF6)水平,探究其与临床指标的关系。方法选取2016年3月-2019年2月在昆山市第一人民医院就诊的慢性无症状HBV携带者(ASC) 、慢性乙型肝炎(CHB)及乙型肝炎肝硬化(LC)患者各20例,收集同期20例体检健康者作为正常对照(NC)。应用实时荧光定量PCR技术检测NC、ASC、CHB及LC组外周血白细胞ATF6 mRNA水平,分析其与临床指标的关系;采用ELISA法检测NC及CHB组血清ATF6蛋白水平。计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验;2组间比较采用独立样本t检验。结果ASC、CHB及LC组患者的外周血白细胞ATF6 mRNA水平分别为1.99±0.70、2.12±0.75、1.97±0.85,均明显高于NC组(0.71±0.26)(P值均<0.05)。ATF6 mRNA表达水平在不同HBsAg水平、HBV DNA定量、转氨酶水平、HBeAg定性及TBil水平各分组间差异均无统计学意义(P值均>005)。CHB组血清ATF6蛋白水平明显高于NC组[(7.66±1.94)ng/ml vs(3.29±0.30)ng/ml,t=-9.971, P<0.001]。结论ATF6在慢性HBV感染者外周血中有着更高的表达,其可能参与慢性HBV感染病程。
【Abstract】:ObjectiveTo investigate the level of activating transcription factor 6 (ATF6) in peripheral blood and its association with clinical indices in patients with chronic HBV infections. MethodsA total of 20 chronic asymptomatic HBV carriers (ASCs), 20 patients with chronic hepatitis B (CHB), and 20 patients with hepatitis B liver cirrhosis (LC), who were treated in The First People′s Hospital of Kunshan from March 2016 to February 2019, were enrolled, and 20 healthy individuals who underwent physical examination during the same period of time were enrolled as normal control group (NC group). Quantitative real-time PCR was used to measure the mRNA expression of ATF6 in peripheral blood leukocytes for all patients, and its correlation with clinical indices was analyzed. ELISA was used to measure the serum level of ATF6 protein for healthy controls and CHB patients. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups; the independent samples t-test was used for comparison between two groups. ResultsThe ASC, CHB, and LC groups had significantly higher mRNA expression of ATF6 in peripheral blood leukocytes than the NC group (1.99±0.70/2.12±0.75/1.97±0.85 vs 0.71±0.26, all P<005). There were no significant differences in the mRNA expression of ATF6 between the individuals with different levels of HBsAg, HBV DNA, aminotransferases, and total bilirubin and with the presence or absence of HBeAg in each group (all P>0.05). The CHB group had a significantly higher serum level of ATF6 protein than the NC group (7.66±1.94 ng/ml vs 3.29±0.30 ng/ml, t=-9.971, P<0001). ConclusionATF6 has a higher expression level in peripheral blood in patients with chronic HBV infection. It may be involved in the course of chronic HBV infection.
【关键字】:乙型肝炎, 慢性; 激活转录因子6; 内质网应激
【Key words】:hepatitis B, chronic; activating transcription factor 6; endoplasmic reticulum stress
【引证本文】:PAN GF, LI GY, JI XL, et al. Measurement and clinical significance of activating transcription factor 6 level in peripheral blood in patients with chronic HBV infection[J]. J Clin Hepatol, 2019, 35(9): 1946-1949. (in Chinese)
潘高峰, 李国云, 季雪良, 等. 慢性HBV感染者外周血ATF6水平检测及其临床意义[J]. 临床肝胆病杂志, 2019, 35(9): 1946-1949.

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