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D-双功能蛋白在大鼠和裸鼠肝细胞癌生长中的作用
Role of D-bifunctional protein in promoting the growth of hepatocellular carcinoma in rats and nude mice
文章发布日期:2019年06月14日  来源:  作者:路欣,刘英,贾蕾,等  点击次数:219次  下载次数:48次

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【摘要】:目的探讨肝细胞癌大鼠模型肝癌组织中D-双功能蛋白(DBP)的表达及DBP诱导裸鼠肿瘤生长情况。 方法22只雄性SD大鼠随机分为2组,正常对照组大鼠8只,腹腔注射2-乙基亚硝胺诱导肝细胞癌模型组大鼠14只,蛋白免疫印迹、免疫组化和逆转录聚合酶链反应检测DBP表达。14只雄性SPF级BALB/c-nu小鼠,随机分为2组,用空质粒和DBP过表达质粒分别转染HepG2细胞,将2组细胞分别注射于裸鼠皮下,空质粒对照组8只,DBP高表达组6只,检测2组裸鼠成瘤的大小。计量资料2组间比较采用t检验。 结果肝细胞癌模型组大鼠肝癌组织中DBP的蛋白表达高于正常大鼠肝脏,差异具有统计学意义(1.10±0.35 vs 0.67±0.12,t=-7.48, P<0.05);mRNA表达也高于正常大鼠肝脏,差异具有统计学意义(3.70±0.85 vs 1.17±0.72,t=-20.46, P<0.05)。DBP过表达组的裸鼠肿瘤体积显著大于空质粒组,差异具有统计学差异[(7590.50±1867.97) mm3 vs (166378±42024) mm3,t=-39.78, P<0.01]。 结论高表达的DBP在大鼠肝癌的发展进程中发挥了促进作用,并且为肝癌的治疗和抑制药物的研发提供新的靶点。
【Abstract】:ObjectiveTo investigate the expression of D-bifunctional protein (DBP) in hepatocellular carcinoma (HCC) tissue in rats and the growth of DBP-induced HCC in nude mice. MethodsA total of 22 male Sprague-Dawley rats were randomly divided into normal control group with 8 rats and model group with 14 rats treated with intraperitoneally injected diethylnitrosamine to induce HCC, and Western blotting, immunohistochemistry, and RT-PCR were used to measure the expression of DBP. A total of 14 specific pathogen-free male BALB/c-nu mice were randomly divided into two groups. HepG2 cells were transfected with empty plasmid or DBP overexpression plasmid and were then injected subcutaneously into nude mice. There were 8 mice in the empty plasmid control group and 6 mice in the DBP high-expression plasmid group, and tumor size was measured for both groups. The t-test was used for comparison of continuous data between groups. ResultsThe rats with HCC had significantly higher protein and mRNA expression of DBP in liver tissue than normal rats (protein: 1.10±0.35 vs 0.67±0.12, t=-7.48, P<0.05; mRNA: 3.70±0.85 vs 1.17±0.72, t=-20.46, P<0.05). The DBP high-expression plasmid group had a significantly higher tumor volume than the empty plasmid group [(7590.50±1867.97)mm3 vs (1663.78±420.24)mm3, t=-39.78, P<0.01]. ConclusionHighly expressed DBP can promote the progression of HCC in rats and thus provides a new target for the treatment of HCC and the research and development of inhibitory drugs.
【关键字】:癌, 肝细胞; D-双功能蛋白;大鼠, Sprague-Dawley; 小鼠, 近交BALB C; 动物实验
【Key words】:carcinoma, hepatocellular; D-bifunctional protein; rats, Sprague-Dawley; mice, inbred BALB C; animal experimentation
【引证本文】:LU X, LIU Y, JIA L, et al. Role of D-bifunctional protein in promoting the growth of hepatocellular carcinoma in rats and nude mice[J]. J Clin Hepatol, 2019, 35(7): 1532-1535. (in Chinese)
路欣,刘英,贾蕾,等. D-双功能蛋白在大鼠和裸鼠肝细胞癌生长中的作用[J]. 临床肝胆病杂志, 2019, 35(7): 1532-1535.

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