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苍术酮对肝癌HepG2细胞活性、凋亡的影响及其相关机制

杨雪丽 薛建华 陈天阳 平键 侯天禄 陈建杰 成扬

杨雪丽,薛建华,陈天阳,等. 苍术酮对肝癌HepG2细胞活性、凋亡的影响及其相关机制[J]. 临床肝胆病杂志, 2021, 37(11): 2589-2594. DOI: 10.3969/j.issn.1001-5256.2021.11.020
引用本文: 杨雪丽,薛建华,陈天阳,等. 苍术酮对肝癌HepG2细胞活性、凋亡的影响及其相关机制[J]. 临床肝胆病杂志, 2021, 37(11): 2589-2594. DOI: 10.3969/j.issn.1001-5256.2021.11.020
YANG XL, XUE JH, CHEN TY, et al. Effect of atractylone on the viability and apoptosis of hepatoma HepG2 cells and related mechanism[J]. J Clin Hepatol, 2021, 37(11): 2589-2594. DOI: 10.3969/j.issn.1001-5256.2021.11.020
Citation: YANG XL, XUE JH, CHEN TY, et al. Effect of atractylone on the viability and apoptosis of hepatoma HepG2 cells and related mechanism[J]. J Clin Hepatol, 2021, 37(11): 2589-2594. DOI: 10.3969/j.issn.1001-5256.2021.11.020

苍术酮对肝癌HepG2细胞活性、凋亡的影响及其相关机制

DOI: 10.3969/j.issn.1001-5256.2021.11.020
基金项目: 

上海市浦东新区卫计委“中医肝病”临床中医特色学科建设项目 PDZY-2018-0607

上海市浦东新区名中医工作室建设项目 PWRzm2020-14

详细信息
    通讯作者:

    成扬,drchengyang@126.com

  • 中图分类号: R735.7

Effect of atractylone on the viability and apoptosis of hepatoma HepG2 cells and related mechanism

Research funding: 

The Project of "Chinese Medicine Liver Disease" Clinical TCM Specialty Construction Project of Shanghai Pudong New Area Health Planning Commission PDZY-2018-0607

The project of Shanghai Pudong New Area Famous Chinese Medicine Studio Construction PWRzm2020-14

  • 摘要:   目的  观察苍术酮对肝癌HepG2细胞活性、凋亡的影响,并探讨其作用机制。  方法  以肝癌HepG2细胞为研究对象,分为苍术酮低、中、高剂量组(5、10、20 μmol/L),对照组加入等体积的DMSO。MMT比色法检测不同浓度苍术酮处理后HepG2细胞活性。流式细胞仪检测HepG2细胞凋亡率及线粒体膜电位。DCFH-DA荧光探针标记法检测HepG2细胞内ROS水平。Transwell实验检测苍术酮对HepG2细胞迁移能力的影响。蛋白质印迹法检测Bcl-2、Bax和Cleaved caspase-3蛋白表达水平。计量资料多组间比较采用单因素方差分析,组间的两两比较采用LSD-t检验。  结果  在苍术酮处理细胞24、48 h后,与对照组同一时间相比,苍术酮低、中、高剂量组的细胞活性呈下降趋势,且差异均有统计学意义(P值均<0.05),苍术酮处理HepG2细胞72 h的半数抑制率为26.19 μmol/L。苍术酮低、中、高剂量组的细胞凋亡率分别为14.34%、29.32%和50.12%,均高于对照组(0.32%)(P值均<0.05)。与对照组比较,苍术酮低、中、高剂量组HepG2细胞中ROS的荧光强度明显升高(P值均<0.05)。在苍术酮处理HepG2细胞48 h后,与对照组比较,苍术酮低、中、高剂量组的细胞迁移数量明显降低(132.67 ± 18.36、57.00 ± 9.26、31.00 ± 2.45 vs 258.11 ± 38.54,P值均<0.05);与对照组比较,苍术酮低、中、高剂量组能显著抑制抗凋亡因子Bcl-2的表达,促进凋亡因子Bax和Cleaved caspase-3的表达,差异均有统计学意义(P值均<0.05)。  结论  苍术酮能够诱导HepG2细胞凋亡,并抑制其迁移,为进一步开发利用苍术酮提供一定的实验基础。

     

  • 图  1  苍术酮对HepG2细胞活性的影响

    注: 与对照组同一时间比较, *P < 0.05。

    图  2  苍术酮对HepG2细胞凋亡率的影响

    图  3  苍术酮对HepG2细胞线粒体膜电位丢失的影响

    注: a, 对照组;b,5 μmol/L组;c, 10 μmol/L组;d, 20 μmol/L组。

    图  4  苍术酮对HepG2细胞中ROS水平的影响

    注: a, 对照组;b,5 μmol/L组;c, 10 μmol/L组;d, 20 μmol/L组,e,苍术酮对HepG2细胞内ROS水平定量分析。

    图  5  结晶紫染色观察苍术酮对HepG2细胞迁移的影响(×200)

    注: a, 对照组;b,5 μmol/L组;c, 10 μmol/L组;d, 20 μmol/L组。

    图  6  苍术酮对HepG2细胞中Bcl-2,Bax和Cleaved caspase-3蛋白表达水平的影响

    表  1  苍术酮对HepG2细胞迁移的影响

    组别 迁移数量
    对照组 258.11±38.54
    5 μmol/L组 132.67±18.361)
    10 μmol/L组 57.00±9.261)2)
    20 μmol/L组 31.00±2.451)2)
    注:与对照组比较,1)P<0.05;与5 μmol/L组比较,2)P<0.05。
    下载: 导出CSV
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