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原发性肝癌常见动物模型的研究进展

夏猛 孙玉浩 王萌 冷静

夏猛,孙玉浩,王萌,等. 原发性肝癌常见动物模型的研究进展[J]. 临床肝胆病杂志, 2021, 37(8): 1938-1942. DOI: 10.3969/j.issn.1001-5256.2021.08.042
引用本文: 夏猛,孙玉浩,王萌,等. 原发性肝癌常见动物模型的研究进展[J]. 临床肝胆病杂志, 2021, 37(8): 1938-1942. DOI: 10.3969/j.issn.1001-5256.2021.08.042
XIA M, SUN YH, WANG M, et al. Research advances in commonly used animal models of primary hepatocellular carcinoma[J]. J Clin Hepatol, 2021, 37(8): 1938-1942. DOI: 10.3969/j.issn.1001-5256.2021.08.042
Citation: XIA M, SUN YH, WANG M, et al. Research advances in commonly used animal models of primary hepatocellular carcinoma[J]. J Clin Hepatol, 2021, 37(8): 1938-1942. DOI: 10.3969/j.issn.1001-5256.2021.08.042

原发性肝癌常见动物模型的研究进展

DOI: 10.3969/j.issn.1001-5256.2021.08.042
基金项目: 

国家自然科学基金地区基金 81860805

国家自然科学基金地区基金 81660753

广西中医药大学歧黄高层次人才团队培育项目 2018001

详细信息
    通讯作者:

    冷静,ljgxy@yahoo.com.cn

  • 中图分类号: R735.7

Research advances in commonly used animal models of primary hepatocellular carcinoma

Funds: 

Regional Foundation of National Natural Science Foundation of China 81860805

Regional Foundation of National Natural Science Foundation of China 81660753

Qihuang High-level Talent Team Cultivation Project of Guangxi University of Chinese Medicine 2018001

  • 摘要: 原发性肝癌是常见的恶性肿瘤之一,由于其治疗上的困难性和复杂性,采用动物模型来研究其发病机制和筛选药物是当前研究的热点,为研究者更清楚了解不同动物模型的特点及适用性。综述了小鼠、大鼠、兔、树鼩等常用的实验动物及建模方法,包括自发型、诱发型、移植型和基因修饰型等,综合文献研究可见,肝癌模型各有特点,当依据实际需要选用合适的模型,其中在中医药研究中,诱发型动物模型较为常用。在模拟人体遗传等因素发病方面,转基因模型无疑是一个很好的模式生物研究平台。为开展肝癌模型研究或者药物筛选研究提供文献参考依据。

     

  • 表  1  不同动物的常用造模方法

    动物 诱发型 移植型 转基因型
    小鼠 DEN和/或CCl4/乙醇的联合诱导[17-18] 腋下注射H22(小鼠肝癌细胞)或将HepG2用微量注射器注入小鼠肝脏[19] 癌基因过表达的c-Myc模型;慢性炎症的TAK1LPC-KO及AlbLTαβ模型[20];HBV转基因模型[21]
    大鼠 DEN和/或CCl4/乙醇的联合诱导;DEN+2-乙酰氨基芴联合诱导[22] 将Walker-256瘤株注入大鼠肝叶实质[23]
    X2瘤株移植接种于兔的肝脏[7]
    树鼩 HBV +黄曲霉毒素B1(AFB1)诱导[24]
    土拨鼠 HV诱导[15]
    斑马鱼 显微注射Hep3B(人肝癌细胞)到斑马鱼卵黄囊中,构建异种移植斑马鱼[25] Xmrk/kras转基因模型、myc转基因模型[26-27]; [HBx,src]与[HBx,src,p53-/+]转基因模型[28]
    下载: 导出CSV
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