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大鼠感染泡球蚴后外周血及脾脏中滤泡辅助T淋巴细胞的变化

张耀刚 李建华 侯静 孙莉 田美媛 江源 黄登亮 张涛 马艳艳 樊海宁

张耀刚, 李建华, 侯静, 等. 大鼠感染泡球蚴后外周血及脾脏中滤泡辅助T淋巴细胞的变化[J]. 临床肝胆病杂志, 2021, 37(7): 1657-1661. DOI: 10.3969/j.issn.1001-5256.2021.07.035
引用本文: 张耀刚, 李建华, 侯静, 等. 大鼠感染泡球蚴后外周血及脾脏中滤泡辅助T淋巴细胞的变化[J]. 临床肝胆病杂志, 2021, 37(7): 1657-1661. DOI: 10.3969/j.issn.1001-5256.2021.07.035
ZHANG YG, LI JH, HOU J, et al. Change in follicular helper T cells in the peripheral blood and spleen of rats after Echinococcus multilocularis infection [J]. J Clin Hepatol, 2021, 37(7): 1657-1661. DOI: 10.3969/j.issn.1001-5256.2021.07.035
Citation: ZHANG YG, LI JH, HOU J, et al. Change in follicular helper T cells in the peripheral blood and spleen of rats after Echinococcus multilocularis infection [J]. J Clin Hepatol, 2021, 37(7): 1657-1661. DOI: 10.3969/j.issn.1001-5256.2021.07.035

大鼠感染泡球蚴后外周血及脾脏中滤泡辅助T淋巴细胞的变化

DOI: 10.3969/j.issn.1001-5256.2021.07.035
基金项目: 

青海大学附属医院中青年项目 ASRF-2018-YB-01

青海省科技厅重大科技专项 2016-SF-A5

国家自然科学基金 81960129

详细信息
    通讯作者:

    马艳艳,mayanyan_research@qhu.edu.cn

    樊海宁,fanhaining@medmail.com.cn

  • 中图分类号: R532.32

Change in follicular helper T cells in the peripheral blood and spleen of rats after Echinococcus multilocularis infection

Funds: 

Qinghai University Affiliated Hospital for Young and Middle-aged Project ASRF-2018-YB-01

Qinghai Provincial Department of Science and Technology Major Science and Technology Project 2016-SF-A5

National Natural Science Foundation of China 81960129

  • 摘要:   目的  分析泡球蚴感染宿主后外周血和脾脏中滤泡辅助T淋巴细胞(Tfh)水平与包虫病进展的关系。  方法  20只SD大鼠随机分为正常对照组和模型组,每组10只,模型组开腹直视下在右肝接种约2000个原头节,对照组不做任何处理,3个月后麻醉处死SD大鼠,取外周血和脾脏细胞,以CD4+CXCR5+PD1+为Tfh细胞的标记,使用流式细胞术检测外周血和脾脏中Tfh细胞的水平。使用t检验比较两组之间Tfh细胞的差异。  结果  大鼠感染泡球蚴3个月后,肝脏可见明显病灶,HE染色显示病灶中可见原头节。泡球蚴感染模型组外周血中CD4+CXCR5+PD1+Tfh细胞在CD4+细胞中的平均占比为25.63%±3.47%,正常对照组为11.12%±2.94%,2组比较差异有统计学意义(t=10.230,P<0.001),模型组CD4+CXCR5+PD1+Tfh细胞在所有细胞中所占的比例较正常对照组下降显著(0.08%±0.02% vs 0.18%±0.05%, t=5.520,P<0.001);在所有细胞中,模型组脾脏中CD4+CXCR5+PD1+Tfh细胞所占的比例(3.00%±0.42%)显著低于正常对照组(5.30%±1.40%)(t=4.769,P<0.001)。  结论  外周血中Tfh细胞与包虫病进展密切相关,有望成为反应泡球蚴感染的指标。

     

  • 图  1  模型建造情况

    注:a, 感染多房棘球蚴3个月后,SD大鼠肝脏病灶情况;b, 病灶HE染色结果(×100)。

    图  2  AE模型鼠外周血中Tfh细胞的流式细胞检测结果

    注:空白组,未标记荧光抗体用于流式细胞术圈门设定界限的组;a、d、g, 外周血中淋巴细胞;b、e、h, CD4+细胞;c、f、i,CD4+中CXCR5和PD1阳性细胞,其中Q2门表示CD4+CXCR5+PD1+Tfh细胞。

    图  3  AE模型鼠脾脏中Tfh细胞的流式细胞检测结果

    注:a、d、g, 目的细胞群;b、e、h, CD4+细胞;c、f、i, CD4+中CXCR5和PD1阳性细胞, 其中Q2门表示CD4+CXCR5+PD1+Tfh细胞。

    表  1  AE模型鼠外周血中Tfh细胞表达情况

    组别 动物数(只) Tfh细胞在CD4+细胞中百分比(%) Tfh细胞在所有细胞中百分比(%)
    正常对照组 10 11.12±2.94 0.18±0.05
    模型组 10 25.63±3.47 0.08±0.02
    t 10.230 5.520
    P <0.001 <0.001
    下载: 导出CSV

    表  2  AE模型鼠脾脏中Tfh细胞表达情况

    组别 动物数(只) Tfh细胞在CD4+细胞中百分比(%) Tfh细胞在所有细胞中百分比(%)
    正常对照组 10 35.82±4.13 5.30±1.40
    模型组 10 33.50±6.40 3.00±0.42
    t 0.974 4.769
    P 0.343 <0.001
    下载: 导出CSV
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  • 收稿日期:  2020-12-10
  • 修回日期:  2021-01-12
  • 刊出日期:  2021-07-20
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