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基于生物信息学分析核仁纺锤体相关蛋白1在肝细胞癌中的表达及对临床预后的影响

靳鑫旻 杨通旺 徐庆国 刘欢 关鸽 臧运金

靳鑫旻, 杨通旺, 徐庆国, 等. 基于生物信息学分析核仁纺锤体相关蛋白1在肝细胞癌中的表达及对临床预后的影响[J]. 临床肝胆病杂志, 2021, 37(7): 1599-1602. DOI: 10.3969/j.issn.1001-5256.2021.07.024
引用本文: 靳鑫旻, 杨通旺, 徐庆国, 等. 基于生物信息学分析核仁纺锤体相关蛋白1在肝细胞癌中的表达及对临床预后的影响[J]. 临床肝胆病杂志, 2021, 37(7): 1599-1602. DOI: 10.3969/j.issn.1001-5256.2021.07.024
JIN XM, YANG TW, XU QG, et al. Expression of nucleolar spindle-associated protein 1 in hepatocellular carcinoma and its effect on clinical prognosis: A bioinformatics analysis[J]. J Clin Hepatol, 2021, 37(7): 1599-1602. DOI: 10.3969/j.issn.1001-5256.2021.07.024
Citation: JIN XM, YANG TW, XU QG, et al. Expression of nucleolar spindle-associated protein 1 in hepatocellular carcinoma and its effect on clinical prognosis: A bioinformatics analysis[J]. J Clin Hepatol, 2021, 37(7): 1599-1602. DOI: 10.3969/j.issn.1001-5256.2021.07.024

基于生物信息学分析核仁纺锤体相关蛋白1在肝细胞癌中的表达及对临床预后的影响

DOI: 10.3969/j.issn.1001-5256.2021.07.024
基金项目: 

国家科技重大专项 2018ZX10302205-005

详细信息
    通讯作者:

    臧运金,zangyj3657@qq.com

  • 中图分类号: R735.7

Expression of nucleolar spindle-associated protein 1 in hepatocellular carcinoma and its effect on clinical prognosis: A bioinformatics analysis

Funds: 

National Science and Technology Major Project 2018ZX10302205-005

  • 摘要:   目的  探讨在肝癌中核仁纺锤体相关蛋白1(NUSAP1)的表达及其对临床预后的影响。  方法  从基因表达数据库(GEO)下载HCC芯片数据集GSE57957、GSE14520、GSE22058、GSE46444、GSE54236、GSE36376、GSE64041、GSE76297、GSE76427、GSE102079和癌症基因组图谱(TCGA)中的HCC RNA-seq数据,利用R 4.0软件分析NUSAP1在肝癌及癌旁组织的表达差异。应用t-分布邻域嵌入算法(tSNE)对单细胞测序数据的降维,分析NUSAP1在肝癌和癌旁组织中的差异表达。收集2018年1月—2019年11月在青岛大学附属医院进行同种异体肝移植术的42例乙型肝炎相关肝癌患者的癌组织及癌旁组织,收集其临床病理资料并分析与NUSAP1基因表达的相关性。计数资料2组间比较采用χ2检验。以Kaplan-Meier方法生存分析NUSAP1表达水平与总体生存率及无病间隔生存率之间的关系,运用log-rank进行检验,通过Graphpad prim 7使其结果可视化。  结果  NUSAP1在GEO数据库与TCGA数据库数据集中的肝癌组织中均高表达(P值均<0.001)。来源于肿瘤组织的细胞其NUSAP1高表达细胞明显高于癌旁组织细胞。NUSAP1在29例患者的肝癌组织中高表达,在13例患者的肝癌组织中低表达,NUSAP1在肝癌组织中高表达组及低表达组间,Child-Pugh分级、TNM分期、Okuda分期比较差异均有统计学意义(χ2值分别为5.469、6.836、4.617,P值均<0.05)。NUSAP1高表达的肝癌患者总体生存率与无病间隔生存率显著低于低表达的肝癌患者,NUSAP1低表达患者1、3、5生存率分别为92.96%、83.80%、76.76%,明显高于高表达的患者(76.76%、67.60%、64.78%),NUSAP1高表达的患者中位生存时间明显低于低表达的患者(61.7个月 vs 108.6个月)(P值均<0.05)。  结论  NUSAP1在肝癌细胞中高表达,可作为乙型肝炎肝癌患者不良预后的潜在标志物。

     

  • 图  1  NUSAP1在临床肝癌样本中高表达

    图  2  TCGA数据库中NUSAP1与总体生存率及无病间隔生存率之间的关系

    表  1  NUSAP1表达水平与肝癌患者临床病理特征的关系

    项目 高表达(n=29) 低表达(n=13) χ2 P
    性别(例) 0.022 0.88
      男 24 11
      女 5 2
    血型(例) 0.628 0.89
      O型 5 3
      A型 11 5
      B型 12 5
      AB型 1 0
    年龄 2.143 0.14
      <50岁 16 4
      ≥50岁 13 9
    BMI 0.633 0.43
      18.5~23.9 kg/m2 14 8
      <18.5 kg/m2或≥24 kg/m2 15 5
    AFP(例) 0.252 0.62
      <20 ng/ml 11 6
      ≥20 ng/ml 18 7
    MELD评分(例) 0.032 0.86
      <20分 17 8
      ≥30分 12 5
    Child-Pugh分级(例) 5.469 0.02
      A级 7 8
      B或C级 22 5
    肿瘤数目(例) 0.001 0.97
      单发 11 5
      多发 18 8
    TNM分期(例) 6.836 0.01
      Ⅰ期 3 6
      Ⅱ或Ⅲ期 26 7
    Okuda分期(例) 4.617 0.03
      Ⅰ 6 7
      Ⅱ或Ⅲ 23 6
    下载: 导出CSV
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  • 收稿日期:  2020-12-14
  • 修回日期:  2021-01-22
  • 刊出日期:  2021-07-20
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