中文English
ISSN 1001-5256
CN 22-1108/R

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

外源性脂联素对内皮素1诱导的肝星状细胞收缩的影响及作用机制

李楠 刘迎娣 郭明洲 梁浩

李楠, 刘迎娣, 郭明洲, 梁浩. 外源性脂联素对内皮素1诱导的肝星状细胞收缩的影响及作用机制[J]. 临床肝胆病杂志, 2016, 32(2): 307-311. DOI: 10.3969/j.issn.1001-5256.2016.02.022
引用本文: 李楠, 刘迎娣, 郭明洲, 梁浩. 外源性脂联素对内皮素1诱导的肝星状细胞收缩的影响及作用机制[J]. 临床肝胆病杂志, 2016, 32(2): 307-311. DOI: 10.3969/j.issn.1001-5256.2016.02.022
Li Nan, Liu YingDi, Guo MingZhou, Liang Hao. Effect of adiponectin on hepatic stellate cell contraction induced by endothelin-1 and its mechanism of action[J]. J Clin Hepatol, 2016, 32(2): 307-311. DOI: 10.3969/j.issn.1001-5256.2016.02.022
Citation: Li Nan, Liu YingDi, Guo MingZhou, Liang Hao. Effect of adiponectin on hepatic stellate cell contraction induced by endothelin-1 and its mechanism of action[J]. J Clin Hepatol, 2016, 32(2): 307-311. DOI: 10.3969/j.issn.1001-5256.2016.02.022

外源性脂联素对内皮素1诱导的肝星状细胞收缩的影响及作用机制

DOI: 10.3969/j.issn.1001-5256.2016.02.022
详细信息
  • 中图分类号: R575.2

Effect of adiponectin on hepatic stellate cell contraction induced by endothelin-1 and its mechanism of action

  • 摘要: 目的观察外源性脂联素对内皮素(ET)1诱导的肝星状细胞(HSC)-T6收缩的影响,探讨脂联素在此过程中的可能作用机制。方法应用胶原晶格法观察不同浓度(0.25、0.5μg/ml)脂联素及脂联素(0.5μg/ml)与左旋硝基精氨酸甲酯(LNAME)(5 mmol/L)共同作用下对ET-1诱导的HSC-T6细胞收缩的影响;分别采用实时荧光定量PCR、Western Blot检测脂联素(0.5μg/ml)作用后HSC-T6细胞中ET-1 mRNA及蛋白的表达,采用ELISA检测脂联素(0.5μg/ml)作用后HSC-T6细胞培养液中ET-1蛋白的表达;采用实时荧光定量PCR及Western Blot检测不同浓度(0.5、1、2μg/ml)脂联素与ET-1共同作用下HSC-T6细胞中诱导型一氧化氮合酶(i NOS)的mRNA及蛋白的表达,同时检测HSC-T6细胞中AMPK、p-AMPK、Akt、p-Akt蛋白的表达。计量资料多组间比较采用方差分析,进一步两两比较采用Dunnett法。结果 (1)胶原晶格实验显示,与空白对照组相比,ET-1组凝胶面积显著收缩[(24.8±7.3)%vs(71.9...

     

  • [1]KHIMJI AK,ROCKEY DC.Endothelin and hepatic wound healing[J].Pharmacol Res,2011,63(6):512-518.
    [2]RODRIGUEZ-PASCUAL F,BUSNADIEGO O,GONZALEZSANTAMARIA J.The profibrotic role of endothelin-1:is the door still open for the treatment of fibrotic diseases?[J].Life Sci,2014,118(2):156-164.
    [3]YOU M,ROGERS CQ.Adiponectin:a key adipokine in alcoholic fatty liver[J].Exp Biol Med(Maywood),2009,234(8):850-859.
    [4]CALIGIURI A,BERTOLANI C,GUERRA CT,et al.Adenosine monophosphate-activated protein kinase modulates the activated phenotype of hepatic stellate cells[J].Hepatology,2008,47(2):668-676.
    [5]NAZAL L,RIQUELME A,SOLIS N,et al.Hypoadiponectinemia and its association with liver fibrosis in morbidly obese patients[J].Obes Surg,2010,20(10):1400-1407.
    [6]LIU Y,RAMEZANI-MOGHADAM M,GEORGE J,et al.Kupffer cells and sinusoidal endothelial cells mediate adiponectin-induced antifibrogenic effects on hepatic stellate cells[J].J Gastroenterol Hepatol,2009,24:a289-a290.
    [7]SEO YS,SHAH VH.Pathophysiology of portal hypertension and its clinical links[J].J Clin Exp Hepatol,2011,1(2):87-93.
    [8]LIM JY,OH MA,KIM WH,et al.AMP-activated protein kinase inhibits TGF-β-induced fibrogenic responses of hepatic stellate cells by targeting transcriptional coactivator p300[J].J Cell Physiol,2012,227(3):1081-1089.
    [9]SHI-WEN X,CHEN Y,DENTON CP,et al.Endothelin-1 promotes myofibroblast induction through the ETA receptor via a rac/phosphoinositide 3-kinase/Akt-dependent pathway and is essential for the enhanced contractile phenotype of fibrotic fibroblasts[J].Mol Biol Cell,2004,15(6):2707-2719.
    [10]OUCHI N,KIHARA S,ARITA Y,et al.Adiponectin,an adipocyte-derived plasma protein,inhibits endothelial NF-κB signaling through a cA MP-dependent pathway[J].Circulation,2000,102(11):1296-1301.
    [11]KIM J,WENDE AR,SENA S,et al.Insulin-like growth factor I receptor signaling is required for exercise-induced cardiac hypertrophy[J].Mol Endocrinol,2008,22(11):2531-2543.
  • 加载中
计量
  • 文章访问数:  2062
  • HTML全文浏览量:  17
  • PDF下载量:  455
  • 被引次数: 0
出版历程
  • 收稿日期:  2015-07-03
  • 出版日期:  2016-02-20
  • 刊出日期:  2016-02-20
  • 分享
  • 用微信扫码二维码

    分享至好友和朋友圈

目录

    /

    返回文章
    返回