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Characterization of peripheral blood lymphocyte subsets in hepatitis C virus-infected patients
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摘要:
目的调查丙型肝炎患者外周血淋巴细胞亚群变化及影响因素。方法利用流式细胞术检测241例丙型肝炎患者和117例健康人群外周血淋巴细胞亚群数值,通过回顾性分析,调查我国丙型肝炎人群淋巴细胞亚群变化及其相关的影响因素。结果在健康人群中,CD3+CD4+T细胞、CD3+CD16+CD56+NKT细胞频率以及CD4/CD8比值与年龄呈显著正相关;而在慢性丙型肝炎人群中,CD3+CD4+T细胞与年龄呈显著正相关,CD3-CD19+B细胞则与年龄呈显著负相关;在肝硬化人群中,只有CD3-CD16+CD56+NK细胞频率与年龄呈显著正相关;在15～49岁的健康人及慢性肝炎人群中,女性CD3+CD4+T细胞亚群频率高于男性,而在肝硬化患者中女性CD3-CD19+B细胞频率低于男性;同时,在HCV感染的不同阶段,CD3-CD16+CD56+NK细胞亚群频率均较正常人显著降低,而CD3+CD8+T细胞频率则显著升高,在15岁以上人群,CD3-CD19+B细胞在健康人、慢性肝炎、肝硬化人群中呈持续升高的现象。结论通过回顾性分析健康人群和HCV感染不同阶段人群淋巴细胞亚群的分布特点及其与HCV疾病进展、年龄、性别...
Abstract:Objective To analyze the peripheral blood lymphocyte subsets in hepatitis C virus (HCV) -infected patients and determine their clinical implications.Methods Peripheral blood samples were collected from 241 patients diagnosed with HCV infection and 117 healthy adult controls between 2001 and 2009.Flow cytometry was used to detect the percentages of lymphocyte subsets in each sample, including CD3+ T cells, CD3+CD4+ T cells, CD3+CD8+ T cells, CD3-CD19+ B cells, CD3-CD16+CD56+ natural killer (NK) cells, and CD3+CD16+CD56+ NKT cells, as well as the CD4/CD8 ratio.Significance of between group differences was assessed by the Mann-Whitney U test.Potential correlation of lymphocyte subset percentages with patient age was analyzed by the Spearman's rank correlation coefficient.Results The percentages of peripheral blood lymphocyte subsets detected in the healthy controls served as the reference standards.In the healthy controls, the frequencies of CD3+CD4+ T cells and CD3+CD16+CD56+ NK cells, and the CD4/CD8 ratio were positively correlated with increasing age.In the HCV-infected patients with chronic hepatitis, the frequency of CD3+CD4+ T cells was also positively correlated with increasing age, but the frequency of CD3-CD19+ B cells was negatively correlated with age.In HCV-infected patients with liver cirrhosis, the frequency of CD3-CD16+CD56+ NK cells was positively correlated with increasing age.In HCV-infected patients between the ages of 15 and 49 years old, the frequency of CD3+CD4+ T cells was higher in females than in males.However, in the sub-group of HCV-infected patients with liver cirrhosis, the frequency of CD3-CD19+ B cells was lower in females than in males.Moreover, compared to the healthy controls, the HCV-infected patients had significantly lower frequency of CD3-CD16+CD56+ NK cells but higher frequency of CD3+CD8+ T cells.HCV-infected patients more than 15 years old and having both chronic hepatitis and liver cirrhosis showed elevated frequency of CD3-CD19+ B cells compared to healthy controls.Conclusion The percentages of peripheral blood lymphocyte subsets are influenced by disease progression in chronic hepatitis C patients;the differential profile may be helpful for evaluating the immune status of HCV-infected subjects in clinic.
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Key words:
- lymphocyte subsets /
- hepatitis C /
- chronic
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