Characterization of peripheral blood lymphocyte subsets in hepatitis C virus-infected patients
Objective To analyze the peripheral blood lymphocyte subsets in hepatitis C virus (HCV) -infected patients and determine their clinical implications.Methods Peripheral blood samples were collected from 241 patients diagnosed with HCV infection and 117 healthy adult controls between 2001 and 2009.Flow cytometry was used to detect the percentages of lymphocyte subsets in each sample, including CD3+ T cells, CD3+CD4+ T cells, CD3+CD8+ T cells, CD3-CD19+ B cells, CD3-CD16+CD56+ natural killer (NK) cells, and CD3+CD16+CD56+ NKT cells, as well as the CD4/CD8 ratio.Significance of between group differences was assessed by the Mann-Whitney U test.Potential correlation of lymphocyte subset percentages with patient age was analyzed by the Spearman's rank correlation coefficient.Results The percentages of peripheral blood lymphocyte subsets detected in the healthy controls served as the reference standards.In the healthy controls, the frequencies of CD3+CD4+ T cells and CD3+CD16+CD56+ NK cells, and the CD4/CD8 ratio were positively correlated with increasing age.In the HCV-infected patients with chronic hepatitis, the frequency of CD3+CD4+ T cells was also positively correlated with increasing age, but the frequency of CD3-CD19+ B cells was negatively correlated with age.In HCV-infected patients with liver cirrhosis, the frequency of CD3-CD16+CD56+ NK cells was positively correlated with increasing age.In HCV-infected patients between the ages of 15 and 49 years old, the frequency of CD3+CD4+ T cells was higher in females than in males.However, in the sub-group of HCV-infected patients with liver cirrhosis, the frequency of CD3-CD19+ B cells was lower in females than in males.Moreover, compared to the healthy controls, the HCV-infected patients had significantly lower frequency of CD3-CD16+CD56+ NK cells but higher frequency of CD3+CD8+ T cells.HCV-infected patients more than 15 years old and having both chronic hepatitis and liver cirrhosis showed elevated frequency of CD3-CD19+ B cells compared to healthy controls.Conclusion The percentages of peripheral blood lymphocyte subsets are influenced by disease progression in chronic hepatitis C patients;the differential profile may be helpful for evaluating the immune status of HCV-infected subjects in clinic.
- lymphocyte subsets /
- hepatitis C /
Sun J, Yu R, Zhu B, et al.Hepatitis C infection and relatedfactors in hemodialysis patients in China:systematic reviewand meta-analysis[J].Ren Fail, 2009, 31 (7) :610-620. Shepard CW, Finelli L, Alter MJ.Global epidemiology of hepatitisC virus infection[J].Lancet Infect Dis, 2005, 5 (9) :558-567. Fung J, Lai CL, Yuen MF.Hepatitis B and C virus-relatedcarcinogenesis[J].Clin Microbiol Infect, 2009, 15 (11) :964-970. Higuchi M, Tanaka E, Kiyosawa K.Epidemiology and clinicalaspects on hepatitis C[J].Jpn J Infect Dis, 2002, 55 (3) :69-77. Barnaba V.Hepatitis C virus infection:a“liaison a trois”amongstthe virus, the host, and chronic low-level inflammation for hu-man survival[J].J Hepatol, 2010, 53 (4) :752-761. Levings MK, Allan S, d’Hennezel E, et al.Functional dy-namics of naturally occurring regulatory T cells in health andautoimmunity[J].Adv Immunol, 2006, 92:119-155. Morishima C, Paschal DM, Wang CC, et al.Decreased NKcell frequency in chronic hepatitis C does not affect ex vivocytolytic killing[J].Hepatology, 2006, 43 (3) :573-580. Zhang Z, Fu JL, Zhou CB, et al.Reference ranges of periph-eral blood lymphocytes subset percentages in hepatitis B vi-rus-infected Chinese population[J].Infect Dis Inform, 2011, 24 (4) :206-210. (in Chinese) 张政, 福军亮, 周春保, 等.中国乙型肝炎患者外周血淋巴细胞亚群频率参考值范围[J].传染病信息, 2011, 24 (4) :206-210. Ding QY, Yu HY, Sun WW, et al.The changes of peripheralblood T-lymphocytes subsets in pediatric severe hepatitis[J].J Clin Hepatol, 2011, 27 (7) :729-730. (in Chinese) 丁巧云, 俞海英, 孙薇薇, 等.小儿重型肝炎患者外周血T淋巴细胞亚群的变化与临床意义[J].临床肝胆病杂志, 2011, 27 (7) :729-730. Denny T, Yogev R, Gelman R, et al.Lymphocyte subsets inhealthy children during the first 5 years of life[J].JAMA, 1992, 267 (11) :1484-1488. Tollerud DJ, Ildstad ST, Brown LM, et al.T-cell subsets inhealthy teenagers:Transition to the adult phenotype[J].Clin Immunol Immunopathol, 1990, 56 (1) :88-96. Utsuyama M, Hirokawa K, Kurashima C, et al.Differentialage-change in the numbers of CD4+CD45RA+and CD4+CD29+T cell subsets in human peripheral blood[J].MechAgeing Dev, 1992, 63 (1) :57-68. Bisset LR, Lung TL, Kaelin M, et al.Reference values forperipheral blood lymphocyte phenotypes applicable to thehealthy adult population in Switzerland[J].Eur J Haematol, 2004, 72 (3) :203-212. Meier A, Chang JJ, Chan ES, et al.Sex differences in the Toll-like receptor-mediated response of plasmacytoid dendriticcells to HIV-1[J].Nat Med, 2009, 15 (8) :955-959. Naugler WE, Sakurai T, Kim S, et al.Gender disparity in liv-er cancer due to sex differences in MyD88-dependent IL-6production[J].Science, 2007, 317 (5834) :121-124. Andrews DM, Estcourt MJ, Andoniou CE, et al.Innate im-munity defines the capacity of antiviral T cells to limit persis-tent infection[J].J Exp Med, 2010, 207 (6) :1333-1343. Novobrantseva TI, Majeau GR, Amatucci A, et al.Attenua-ted liver fibrosis in the absence of B cells[J].J Clin Invest, 2005, 115 (11) :3072-3082.
- 文章访问数: 2675
- HTML全文浏览量: 18
- PDF下载量: 633
- 被引次数: 0