当飞利肝宁胶囊改善高脂饮食联合四氯化碳诱导的大鼠非酒精性脂肪性肝炎的作用机制

宋海燕; 毛志敏; 杨丽丽; 张莉; 葛迎利; 柳涛; 刘平; 季光

当飞利肝宁胶囊改善高脂饮食联合四氯化碳诱导的大鼠非酒精性脂肪性肝炎的作用机制

1. 上海中医药大学脾胃病研究所上海中医药大学附属龙华医院消化内科
2. 上海高校中医内科学E-研究院

基金项目:

上海市教育委员会重点学科建设项目(J50305);上海市教委预算内科研项目(2010JW35)；上海高校创新团队建设项目(第一期)；

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中图分类号: R285.5
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出版历程
- 出版日期: 2012-03-20

Mechanism of Dangfei Liganning Capsule attenuating nonalcoholic steatotic hepatitis induced by high-fat diet combined with carbon tetrachloride in rats

Research funding:

摘要

摘要: 目的研究当飞利肝宁胶囊对高脂饮食联合四氯化碳(CCl4)诱导的大鼠非酒精性脂肪性肝炎(NASH)防治作用及机制。方法雄性Wistar大鼠,随机分为正常对照组、NASH模型组和当飞利肝宁胶囊治疗组。正常组大鼠喂食普通饲料,模型和治疗组给予高脂饲料,治疗组同时每天予当飞利肝宁胶囊灌胃干预。8周后,除正常组外,其余大鼠腹腔注射CCl4,48 h后处死并收集血清和肝脏组织。观察肝脏组织病理学改变,检测血清ALT、AST、乳酸脱氢酶(LDH)、总胆红素(TBil)、胆固醇(TC)、甘油三脂(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和游离脂肪酸(FFA)水平,检测血清空腹血糖(FBG)、空腹胰岛素(FIN)并计算HOMA指数,以及血清脂联素和肝组织肿瘤坏死因子(TNFα)含量。结果肝组织油红O染色显示,模型组出现大面积小叶内肝细胞脂肪变性,HE染色同时显示出肝细胞气球样变性和炎性细胞浸润,而治疗组肝细胞脂肪变性、气球样变和炎性浸润明显减轻。模型组血清ALT、AST、LDH、TG、LDL、FFA水平较正常组显著升高(P<0.05),HOMA指数和肝组织TNFα含量也显著升高(P&...
- 脂肪肝,非酒精性 /
- 当飞利肝宁胶囊 /
- 胰岛素抵抗 /
- 脂联素 /
- 肿瘤坏死因子α
Abstract: Objective To study the therapeutic effect and potential mechanism of Dangfei Liganning Capsules on nonalcoholic steatotic hepatitis (NASH) induced by high-fat diet and carbon tetrachloride in rats.Methods Male Wistar rats were randomly divided into normal group, model group and treatment group.The rats of normal group were fed with a standard diet, while the rats of model and treatment groups were fed with high-fat diet.In addition the rats of treatment group were gastrically perfused with Dangfei Liganning Capsules everyday.Rats from model and treatment groups were intraperitoneally injected with CCl4 48 h before being sacrificed.The pathology of liver and ALT, AST, LDH, TBIL, TC, TG, HDL, LDL, FFA in serum were tested.The fasting blood glucose (FBG) and fasting insulin (FIN) were tested to calculate HOMA index.Serum adiponectin and hepatic levels of TNF α were assessed.Results Compared with normal group, model group showed massive steatosis with Oil Red-O stain in liver lobules with hepatocyte ballooning and inflammatory infiltration with H-E stain appeared in centrilobules, which were all improved in the treatment group.Levels of serum ALT, AST, LDH, TG, LDL and FFA increased dramatically in model rats, HOMA index and TNF α content in liver tissue also elevated.Serum adiponectin decreased dramatically (P<0.05) .The above examined indexes in treatment group were improved obviously (P<0.05) .Conclusion Dangfei Liganning Capsules could alleviate high fat-diet combined with toxin induced NASH in rats, and the regulation of adiponectin and TNF α level, as well as attenuating insulin resistance contribute to the underlying mechanism.
- fatty liver /
- non-alcoholic /
- Dangfei Liganning Capsule /
- insulin resistance /
- adiponectin

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参考文献(28)

[1]Caldwell S, Argo C.The natural history of non-alcoholic fat-ty liver disease[J].Dig Dis, 2010, 28 (1) :162-168.

[2]Bellentani S, Scaglioni F, Marino M, et al.Epidemiology ofnon-alcoholic fatty liver disease[J].Dig Dis, 2010, 28 (1) :155-161.

[3]Fan JG, Farrell GC.Epidemiology of non-alcoholic fatty liverdisease in China[J].J Hepatol, 2009, 50 (1) :204-210.

[4]Adams LA, Lymp JF, St SJ, et al.The natural history ofnonalcoholic fatty liver disease:a population-based cohortstudy[J].Gastroenterology, 2005, 129 (1) :113-121.

[5]黄欣, 张哲永.当飞利肝宁胶囊治疗非酒精性脂肪性肝病32例临床观察[J].中医杂志, 2007, 48 (6) :524-525.

[6]张雳.当飞利肝宁胶囊治疗脂肪肝257例临床观察[J].中医杂志, 2005, 46 (4) :276.

[7]吴佳玉, 江南, 黄仁刚.当飞利肝宁治疗非酒精性脂肪性肝炎的临床研究[J].四川省卫生管理干部学院学报, 2008, 27 (3) :192-193.

[8]武敬, 彭雁忠.当飞利肝宁胶囊对酒精性脂肪肝患者疗效分析[J].医学信息 (中旬刊) , 2010, 5 (5) :1252.

[9]丁葆英.当飞利肝宁胶囊治疗慢性乙型肝炎45例[J].中医杂志, 2009, 50 (4) :367.

[10]宋毅, 刘培曦.当飞利肝宁防治抗结核药致肝损害的效果观察[J].中国现代药物应用, 2011, 5 (3) :13-14.

[11]Kucera O, Lotkova H, Stankova P, et al.Is rat liver affectedby non-alcoholic steatosis more susceptible to the acutetoxic effect of thioacetamide?[J].Int J Exp Pathol, 2011, 92 (4) :281-289.

[12]Donthamsetty S, Bhave VS, Mitra MS, et al.Nonalcoholicfatty liver sensitizes rats to carbon tetrachloride hepatotoxicity[J].Hepatology, 2007, 45 (2) :391-403.

[13]Lackner C.Hepatocellular ballooning in nonalcoholic steato-hepatitis:the pathologist's perspective[J].Expert Rev Gas-troenterol Hepatol, 2011, 5 (2) :223-231.

[14]周大成, 朴惠善, 秦文杰, 等.当药的研究进展[J].时珍国医国药, 2011, 22 (2) :462-465.

[15]周大成, 朴惠善, 张思玉, 等.当药提取物对鼠试验性肝损伤的保护作用[J].中国实验方剂学杂志, 2010, 16 (17) :125-128.

[16]王玉刚, 施敏, 陈锡美, 等.水飞蓟素治疗大鼠非酒精性脂肪性肝炎的机制探讨[J].中华消化杂志, 2008, 28 (4) :263-265.

[17]段钟平, 朱跃科, 陈煜, 等.水飞蓟宾磷脂复合物防治脂肪肝的动物试验与临床观察[J].实用肝脏病杂志, 2008, 11 (4) :218-221.

[18]程勇, 苏剑, 田艳, 等.水飞蓟宾胶囊对非酒精性脂肪性肝病患者血清IL-8、TNFα的影响[J].山东医药, 2009, 49 (7) :62-63.

[19]陈黎, 陈枝俏.当飞利肝宁胶囊联合血脂康治疗合并高脂血症的非酒精性脂肪性肝病[J].中国中医药信息杂志, 2007, 14 (2) :64-65.

[20]刘芳, 谢贤春, 吉中和, 等.当飞利肝宁胶囊治疗高脂血症及脂肪肝的实验研究[J].医学理论与实践, 2007, 20 (3) :251-253.

[21]黄久荣, 梁若梅.当飞利肝宁胶囊在降脂治疗中的保肝作用观察[J].中医杂志, 2007, 48 (7) :598.

[22]Garcia-Monzon C, Lo IO, Mayoral R, et al.Hepatic insulinresistance is associated with increased apoptosis and fibro-genesis in nonalcoholic steatohepatitis and chronic hepatitis C[J].J Hepatol, 2011, 54 (1) :142-152.

[23]Ratziu V, Caldwell S, Neuschwander-Tetri BA.Therapeutictrials in nonalcoholic steatohepatitis:insulin sensitizers andrelated methodological issues[J].Hepatology, 2010, 52 (6) :2206-2215.

[24]杨玉锋, 南月敏.脂联素与非酒精性脂肪性肝病的研究进展[J].临床肝胆病杂志, 2009, 25 (2) :155-157.

[25]Polyzos SA, Kountouras J, Zavos C.The role of adiponectinin the pathogenesis and treatment of non-alcoholic fatty liv-er disease.[J].Diabetes Obes Metab, 2010, 12 (5) :365-383.

[26]Savvidou S HPOH.Low serum adiponectin levels are predic-tive of advanced hepatic fibrosis in patients with NAFLD[J].J Clin Gastroenterol, 2009, 43 (8) :765-772.

[27]Fukushima J, Kamada Y, Matsumoto H, et al.Adiponectinprevents progression of steatohepatitis in mice by regulatingoxidative stress and Kupffer cell phenotype polarization[J].Hepatol Res, 2009, 39 (7) :724-738.

[28]Tilg H.The role of cytokines in non-alcoholic fatty liver dis-ease[J].Dig Dis, 2010, 28 (1) :179-185.

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