调节性T细胞对肝星状细胞增殖及透明质酸分泌的影响

陆小蒟; 陈永平; 阳韬; 程瑗; 金晓芝; 申春燕; 刘俊; 王春莹; 王晓东; 林镯

调节性T细胞对肝星状细胞增殖及透明质酸分泌的影响

温州医学院附属第一医院

基金项目:

浙江省自然科学基金资助项目(Y207464)；浙江省教育厅项目(Y201009942)；中国肝炎防治基金会王宝恩肝纤维化研究基金(20080017)；

详细信息

中图分类号: R575.1
计量
- 文章访问数: 3116
- HTML全文浏览量: 20
- PDF下载量: 721
- 被引次数: 0
出版历程
- 出版日期: 2012-01-20

The influence of regulatory T cells in hepatic stellate cell proliferation and HA secretion

Research funding:

摘要

摘要: 目的研究CD4+CD25+调节性T细胞（Treg）对肝星状细胞（HSC）增殖及透明质酸（HA）分泌的影响。方法 MASC法分离慢性乙型肝炎患者血中CD4+CD25+Treg细胞;传代培养人HSC LX-2（空白组）,不同比例CD4+CD25+Treg细胞与HSC直接共培养（共培养组）,不同剂量的重组人转化生长因子（TGF）β1和HSC共培养（对照组）;使用CCK-8法检测HSC增殖、放射免疫法检测HA分泌情况。组间比较采用单因素方差分析。结果共培养组与空白对照组（0.6623±0.02702）相比,在比例为2.5∶1时HSC增殖最为明显（0.6836±0.00560）,比例为1∶1、5∶1及7.5∶1时HSC增殖均明显减少,且随着比例升高其增殖水平逐渐降低,各组与空白对照组相比有统计学差异（F=11.421,5.272,84.514,490.639;均P<0.05）。不同浓度TGFβ1对照组的HSC均比空白对照组（0.3883±0.00189）细胞增殖明显（0.6720±0.00753,0.6773±0.00585,0.5373±0.00387）,并于8 ng/μl时增殖达到高峰...
- T淋巴细胞 /
- 肝星状细胞 /
- 透明质酸,细胞增殖
Abstract: Objective To investigate the effect of CD4+CD25+ regulatory T cells (Treg) in the proliferation of hepatic stellate cells (HSC) and the secretion of Hyaluronic Acid (HA) .Methods By using magnetic activated cell sorter (MRCS) , Treg cells were isolated from peripheral blood of chronic hepatitis B patients The hepatic stellate cells (HSC) cultured alone were used as blank group.The LX-2 were co-cultured with Treg cells in vitro as co-culture group;adding TGFβ1 to HSC co-cultured as control group.CCK-8 method for the detection of the proliferation of HSC, ELISA analysis for the detection of HA secretion was performed.One-way analysis of variance was used for the comparison.Results Compared with the blank group (0.6623±0.02702) the proliferation of HSC of the co-culture group was significantly higherand the most prominent effect was 2.5∶ 1 (0.6836±0.00560) , while the proliferation of HSC decreased at 1∶ 1、5∶ 1 and 7.5∶ 1, and the discrepancy between co-culture subgroups and blank group had statistical significance (F=11.421, 5.272, 84.514, 490.639;P<0.05) .Compared with blank group, the proliferation of HSCs of different concentrations of TGFβ1 control subgroup increased significantly (0.6720±0.00753, 0.6773±0.00585, 0.5373±0.00387) and reached a peak at 8 ng/μl, and the discrepancy between control subgroups and blank group had statistical significance (F=196.175, 799.624, 236.461;P<0.01) .Linear correlation analysis showed concentrations of HA and the proliferation of HSC in co-culture group was positively correlated with control group (r=0.661, 0.871;P<0.01) .Conclusion CD4+CD25+ Treg cells could accelerate the cultivation of HSCs proliferation and HA secretion.And, in vitro, we confirmed CD4+CD25+ Treg cells play an important role in the process of chronic hepatitis B.
- T-lymphocytes /
- hepatic stellate cells /
- hyaluronic acid

HTML全文

参考文献(12)

[1]Wells RG.Liver fibrosis:challenges of the new era[J].Gastroenterology, 2009, 136 (2) :387-388.

[2]Vinas O, Bataller R, Sancho-Bru P, et al.Human hepatic stellate cells show features of antigen-presenting cells and stimulate lymphocyte proliferation[J].Hepatology, 2003, 38 (4) :919-929.

[3]中华医学会.病毒性肝炎防治方案[J].中华传染病杂志, 2001, 19 (1) :56-62.

[4]Manning DS, Afdhal NH.Diagnosis and quantitation off ibro-sis[J].Gastroenterology, 2008, 134 (6) :1670-1681.

[5]Winau F, Hegasy G, Weiskirchen R, et al.II to cells are liver-resident antigen-presenting cells for activating T cell re-sponses[J].Immunity, 2007, 26 (1) :117-129.

[6]akaguchi S, Sakaguchi N, Asano M, et al.Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25) .Breakdown of a single mech-anism of self-tolerance causes various autoinunune disea-ses[J].J Inmunol, 1995, 155 (3) :1151-1164.

[7]Fontenot JD, Rudensky AY.A well adapted regulatory con-trivance:regulatory Tcell development and the forkhead fami-ly transcription factor Foxp3[J].Nat Immunol, 2005, 6 (4) :331-337.

[8]Seddiki N, Santner-Nanan B, Martinson J, et al.Expres-sion of interleukin (IL) -2 and IL-7 receptors discrimi-nates between human regulatory and activated T cells[J].J Exp Med, 2006, 203 (7) :1693-1700.

[9]Liu W, Putnam AL, Xu-Yu Z, et al.CD127 expression in-versely correlates with FoxP3 and suppressive function of hu-man CD4+ Treg cells[J].J Exp Med, 2006, 203 (7) :1701-1711.

[10]Belkaid Y, Piccirillo CA, Mendez S, et al.CD4+CD25+regu-latory T cel1s control Leishmania major persistence and im-munity[J].Nature, 2002, 420 (6915) :502-507.

[11]Boettler T, Spangenberg HC, Neumann-Haefelin C, et al.T cells witha CD4+CD25+regulatory phenotype suppress in vitro proliferation of virus-specific CD8+T cells during chro-nic hepatitis C virus infection[J].J Virol, 2005, 79 (12) :7860-7867.

[12]Franzese O, Kennedy PT, Gehring AJ, et al.Modulation of the CD8+-T-cell response by CD4+CD25+regulatory T cells inpatients with hepatitis B virus infection[J].J virol, 2005, 79 (6) :3322-3328.

施引文献

资源附件(0)

访问统计