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Tet-on和Cre/loxP双调控肝靶向表达Cre重组酶转基因小鼠的制备和功能评价

赵亚 马玉 黄豫晓 兰海云 孙梦宁 吕欣 杨敬 雷迎峰 张建敏 康健 招明高 汪爱勤 尹文

赵亚, 马玉, 黄豫晓, 兰海云, 孙梦宁, 吕欣, 杨敬, 雷迎峰, 张建敏, 康健, 招明高, 汪爱勤, 尹文. Tet-on和Cre/loxP双调控肝靶向表达Cre重组酶转基因小鼠的制备和功能评价[J]. 临床肝胆病杂志, 2011, 27(1): 81-83.
引用本文: 赵亚, 马玉, 黄豫晓, 兰海云, 孙梦宁, 吕欣, 杨敬, 雷迎峰, 张建敏, 康健, 招明高, 汪爱勤, 尹文. Tet-on和Cre/loxP双调控肝靶向表达Cre重组酶转基因小鼠的制备和功能评价[J]. 临床肝胆病杂志, 2011, 27(1): 81-83.
Zhao Ya, Ma Yu, Huang YuXiao, Lan HaiYun, Sun MengNing, Lu: Xin, Yang Jing, Lei YingFeng, Zhang JianMin, Kang Jian, Zhao MingGao, Wang AiQin, Yin Wen. Functional assessment of expression of Cre recombinase targeting the liver of transgenic mice regulated by Tet-on and Cre/loxP system[J]. J Clin Hepatol, 2011, 27(1): 81-83.
Citation: Zhao Ya, Ma Yu, Huang YuXiao, Lan HaiYun, Sun MengNing, Lu: Xin, Yang Jing, Lei YingFeng, Zhang JianMin, Kang Jian, Zhao MingGao, Wang AiQin, Yin Wen. Functional assessment of expression of Cre recombinase targeting the liver of transgenic mice regulated by Tet-on and Cre/loxP system[J]. J Clin Hepatol, 2011, 27(1): 81-83.

Tet-on和Cre/loxP双调控肝靶向表达Cre重组酶转基因小鼠的制备和功能评价

详细信息
  • 中图分类号: R512.63

Functional assessment of expression of Cre recombinase targeting the liver of transgenic mice regulated by Tet-on and Cre/loxP system

  • 摘要:

    目的制备Tet-on和Cre/loxP系统双重调控下肝靶向性表达Cre重组酶的转基因小鼠rtTALAP-1/LC-1并评价其功能,为最终建立可突破胚胎期免疫耐受的双调控型HCV转基因小鼠模型奠定基础。方法选取适龄rtTALAP-1转基因小鼠与LC-1转基因小鼠交配,PCR法检测子代rtTALAP-1/LC-1转基因小鼠基因组中是否插入了rtTA元件和Cre基因片段。双阳性rtTALAP-1/LC-1小鼠dox诱导1周后,以小动物活体成像系统检测小鼠肝脏的Luc萤光素酶信号,免疫组化检测Cre重组酶在小鼠肝脏等组织中的表达状况。结果 rtTALAP-1/LC-1转基因小鼠在dox诱导后,仅在其肝脏检测到清晰的Luc萤光素酶信号,而其他脏器均未检测到萤光信号;免疫组化法也仅在小鼠肝细胞核中检测到Cre重组酶的表达,心脏、肾脏和骨骼肌等其他组织均未检测到Cre重组酶的表达。结论成功制备了rtTALAP-1/LC-1转基因小鼠,其靶基因表达的诱导反应性和肝靶向性均良好,为最终制备双调控型丙型肝炎病毒(HCV)转基因小鼠模型奠定了良好的基础。

     

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  • 出版日期:  2011-01-20
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