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荧光RT-PCR法检测丙型肝炎病毒基因型

郭新会 严艳 李卓 殷继明

郭新会, 严艳, 李卓, 殷继明. 荧光RT-PCR法检测丙型肝炎病毒基因型[J]. 临床肝胆病杂志, 2011, 27(1): 65-67.
引用本文: 郭新会, 严艳, 李卓, 殷继明. 荧光RT-PCR法检测丙型肝炎病毒基因型[J]. 临床肝胆病杂志, 2011, 27(1): 65-67.
Guo XinHui, Yan Yan, Li Zhuo, Yin JiMing. Fluorescent reverse transcription-PCR assay for determination of hepatitis C virus genotypes[J]. J Clin Hepatol, 2011, 27(1): 65-67.
Citation: Guo XinHui, Yan Yan, Li Zhuo, Yin JiMing. Fluorescent reverse transcription-PCR assay for determination of hepatitis C virus genotypes[J]. J Clin Hepatol, 2011, 27(1): 65-67.

荧光RT-PCR法检测丙型肝炎病毒基因型

详细信息
  • 中图分类号: R512.63

Fluorescent reverse transcription-PCR assay for determination of hepatitis C virus genotypes

  • 摘要:

    目的应用荧光RT-PCR方法对慢性丙型肝炎(CHC)患者血清进行HCV基因型分析。方法采用荧光RT-PCR方法与测序方法比较,对118例CHC阳性患者血清进行HCV RNA分型,比较两种方法分型结果的一致性;为了考察该方法的特异性,对98例慢性乙型肝炎(CHB)患者血清进行HCV RNA分型检测。结果在118例HCV RNA阳性血清标本中,用荧光RT-PCR方法检测有HCV基因1型79例(66.9%),2型29例(24.6%),1/2混合型8例(6.8%);另有2例(1.7%)HCV RNA定量为1×103IU/ml弱阳性标本未分出型。测序法分出1型81例(68.4%),2型29例(24.6%),1/2混合型6例(5.1%),与荧光RT-PCR方法一致2例(1.7%)HCV RNA定量结果为1×103IU/ml的阳性标本测序法未分出型。未发现其他基因型的病例。98例CHB患者血清检测结果均为阴性。结论荧光HCV基因分型的方法具有良好的敏感性、特异性、可重复性且省时省力,可选择该方法为临床诊断服务。

     

  • [1]中华医学会.丙型肝炎防治指南[J].中华肝脏病杂志, 2004, 12 (4) :194-198.
    [2]Simmonds P, Holmes EC, Cha TA, et al.Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region[J].J Gen Virol, 1993, 74 (11) :2391-2399.
    [3]Moreno C, Deltenre P, Pawlotsky JM, et al.Shortened treatment duration in treatment-naive genotype 1 HCV patients with rapid virological response:a meta-analysis[J].J Hepatol, 2010, 52 (1) :25-31.
    [4]Mangia A, Santoro R, Andriulli A, et al.Peginterferon alfa-2b and ribavirin for 12 vs.24 weeks in HCV genotype 2 or 3[J].N Engl J Med, 2005, 352 (25) :2609-2617.
    [5]Davis GL, Wong JB, McHutchison JG, et al.Early virologic response to treatment with peginterferon alfa-2b plus ribavirin in patients with chronic hepatitis C[J].Hepatology, 2003, 38 (3) :645-652.
    [6]Wong JB, Davis GL, McHutchinson JG, et al.Clinical implications of testing viral response during ribavirin and peginterferon alfa-2b treatment for chronic hepatitis C[J].Hepatology, 2002, 36:281A.
    [7]Bullock C, Bruns E, Haverstick DM, et al.Hepatitis C genotype determination by melting curve analysis with a single set of fluorescent resonance energy transfer probes[J].Clin Chem, 2002, 48 (12) :2147-2154.
    [8]Fujigaki H, Takemura M, Takahashi K, et al.Genotyping of hepatitis C virus by melting curve analysis with SYBR green I[J].Ann Clin Biochem, 2004, 41 (Pt2) :130-132.
    [9]Linda C, Ka WS, Elizabeth M, et al.Mul-tiplex Real-Time Reverse Transcription-PCR Assay for Determination of Hepatitis C Virus Genotypes[J].J Clin Microbiol, 2006, 41 (11) :1091-1100.
    [10]Rolfe KJ, Alexander GJ, Wreghitt TG, et al.A real-time Taqman method for hepatitis C virus genotyping[J].J Clin Virol, 2005, 34 (2) :115-121.
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  • 出版日期:  2011-01-20
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