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荧光RT-PCR法检测丙型肝炎病毒基因型

郭新会 严艳 李卓 殷继明

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荧光RT-PCR法检测丙型肝炎病毒基因型

详细信息
  • 中图分类号: R512.63

Fluorescent reverse transcription-PCR assay for determination of hepatitis C virus genotypes

  • 摘要:

    目的应用荧光RT-PCR方法对慢性丙型肝炎(CHC)患者血清进行HCV基因型分析。方法采用荧光RT-PCR方法与测序方法比较,对118例CHC阳性患者血清进行HCV RNA分型,比较两种方法分型结果的一致性;为了考察该方法的特异性,对98例慢性乙型肝炎(CHB)患者血清进行HCV RNA分型检测。结果在118例HCV RNA阳性血清标本中,用荧光RT-PCR方法检测有HCV基因1型79例(66.9%),2型29例(24.6%),1/2混合型8例(6.8%);另有2例(1.7%)HCV RNA定量为1×103IU/ml弱阳性标本未分出型。测序法分出1型81例(68.4%),2型29例(24.6%),1/2混合型6例(5.1%),与荧光RT-PCR方法一致2例(1.7%)HCV RNA定量结果为1×103IU/ml的阳性标本测序法未分出型。未发现其他基因型的病例。98例CHB患者血清检测结果均为阴性。结论荧光HCV基因分型的方法具有良好的敏感性、特异性、可重复性且省时省力,可选择该方法为临床诊断服务。

     

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  • 出版日期:  2011-01-20
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