Home   About the Journal  Editorial Board  Online Journal   Publishing Ethics   Contact Us  Chinese Version
You are here:Home => Online Journal => 7期肝癌的诊断和治疗 => 肝脏肿瘤 => 血管内皮生长因子C靶向分子探针在肝细胞癌大鼠模型中的特异性磁共振成像及临.
Specific magnetic resonance imaging of vascular endothelial growth factor-C targeted molecular probe and its clinical significance in a rat model of hepatocellular carcinoma
文章发布日期:2018年06月07日  来源:  作者:潘奇,罗春海,马婉玲,等  点击次数:611次  下载次数:88次
Adjust font size:Small regular big

【Abstract】:Objective To investigate the magnetic resonance (MR) imaging features of the targeted molecular probe with vascular endothelial growth factor-C (VEGF-C) antibody and superparamagnetic iron oxide (USPIO), VEGF-C-USPIO, in a rat model of hepatocellular carcinoma (HCC) and its clinical significance. Methods The induction method was used to establish a rat model of in situ HCC, and 30 Sprague-Dawley rats were randomly divided into experimental group with 20 rats and control group with 10 rats. The rats in the experimental group were treated with tail vein injection of the targeted molecular probe VEGF-C-USPIO, and those in the control group were treated with tail vein injection of the non-targeted probe USPIO. MR scanning was performed before injection and at 1 hour after injection; the intensity of T2WI signal in liver tumor and adjacent liver tissue was measured; contrast-to-noise ratio (CNR) was calculated, and the two groups were compared in terms of CNR before and after enhancement. Liver tissue was collected after scanning, and HE staining was performed to clarify the pathological type of liver cancer in rats; Prussian blue staining was performed to analyze the content of iron in tumor cells; immunohistochemical staining was performed to investigate the expression of VEGF-C in liver cancer tissue. The independent samples t-test was used for comparison between the experimental group and the control group, and the paired samples t-test was used for comparison within each group after the injection of contrast agent. Results Cancer was successfully induced in all 30 rats; the pathological diagnosis was HCC, and the tumor formation rate was 100%. The experimental group had a significant change in CNR at 1 hour after the injection of the targeted contrast agent VEGF-C-USPIO (2.11±0.23 vs 3.47±0.45, t=-13.15, P<0.001), while the control group had no significant change in CNR at 1 hour after the injection of the non-targeted contrast agent USPIO (3.51±0.14 vs 3.82±0.61, t=-1.40, P=0.192); there was a significant difference in CNR between the two groups after injection (t=17.60, P<0.001). HE staining performed for liver tissue samples showed a pathological type of HCC; immunohistochemical staining showed that VEGF-C was mainly expressed in the membrane and cytoplasm of hepatoma cells; Prussian blue staining showed that compared with the control group, the experimental group had a significant increase in iron particles in tumor tissue. Conclusion The synthesized targeted molecular probe VEGF-C-USPIO has a good active targeting effect on a rat model of HCC and can realize the specific imaging of HCC through the change in MR signal intensity. Therefore, it provides an imaging basis for the early diagnosis of HCC.
【Key words】:carcinoma, hepatocellular; vascular endothelial growth factor C; magnetic resonance imaging; ultrasmall superparamagnetic iron oxide nanoparticles; rats, Sprague-Dawley

Mail address: Editorial Office of Journal of Clinical Hepatology, 519 Dongminzhu Street, Changchun, Jilin, China Postal code: 130061 Tel:+86-431-88782542/3542
Journal of Clinical Hepatology Copyright © 2009 - 2013 Lcgdbzz.org. All Rights Reserv 吉ICP备10000617号