【Abstract】：Objective To investigate the association of N-glycosylation mutation of HBV envelope protein with the development of acute-on-chronic liver failure (ACLF) in patients with chronic hepatitis B (CHB). Methods A total of 90 patients with HBV-ACLF were enrolled, and 60 patients with CHB were enrolled as control group. Nested PCR was used for the multiplication and sequencing of HBV S region, and the association of N-glycosylation of HBV envelope protein with the development ACLF was analyzed, as well as the association of N-glycosylation of HBV envelope protein with the severity and 90-day survival rate of ACLF. Results A total of 51 CHB patients (85.0%) and 79 HBV-ACLF patients (87.8%) were included in analysis. Among the CHB patients, 33 (64.7%) had Asn-59 N-glycosylation and 21 (41.2%) had Asn-4 N-glycosylation, while among the HBV-ACLF patients, 16 (20.3%) had Asn-59 N-glycosylation and 14 (17.7%) had Asn-4 N-glycosylation; there were significant differences in the numbers of cases of Asn-59 and Asn-4 N-glycosylation between the two groups (P＜0.001, and P=0.003). There were no significant differences in liver function, HBV DNA, Model for End-Stage Liver Disease score, and 90-day survival rate between the ACLF patients with Asn-59 and Asn-4 N-glycosylation and those without such glycosylation (P＞0.05). Conclusion The reduction in Asn-59 and Asn-4 N-glycosylation induced by HBV envelope protein variation may be associated with the development of ACLF.
【Key words】：hepatitis B virus; liver failure; viral envelope proteins; glycosylation