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胎肝提取物对BEL-7402肝癌细胞的影响
Effect of Embryohepatic Extractions on Hepatoma BEL7402 Cells in Cultures
文章发布日期:2009年10月27日  来源:  作者:  点击次数:5894次  下载次数:1990次
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【摘要】:从4个月胎肝中制备提取物,采用3HTdR掺入、MTT比色法和细胞形态学等技术观察了胎肝提取物(ELE)对BEL7402肝癌细胞的影响。1%和2%DMSO对细胞DNA合成的抑制率为56%和56%;对线粒体脱氢酶的抑制率为43%和48%;形态学改变主要表现为有丝分裂相减少,胞质伸出粗细不等的突起,相互连成网状结构。50500mg/L的EHE可明显抑制7402细胞的DNA合成,并呈剂量作用效应,IC50为240mg/L;对线粒体脱氢酶活力的影响,500mg/L时有轻微的抑制,而50mg/L则有一定的促进作用;对7402细胞形态改变与DMSO作用后分化的形态改变相似。以上结果提示,ELE可抑制7402细胞的增殖并促进其分化。
【Abstract】:mbryohepatic extractions(EHE)were extracted from 4 month embryohepatic tissue.Effect of EHE and dimethylsulfoxide (DMSO)on BEL7402 cells were investigated by using 3 HTdR incorporation,MTT colorimetry method and morphological observation.The results showed that EHE can apparantly inhibit the DNA synthesis and proliferation of BEL7402 cells,IC50 is 240 mg/L.Both EHE and DMSO can induce BEL7402 cells to differentiate morphologically.
【关键字】:胎肝提取物; 肝癌细胞; 肝细胞分化因子; 二甲亚砜(DMSO)
【Key words】:Hepatoma Differentiation Growth inhibitors
【引证本文】:

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